Rat Anti-Mouse Tgfbr1 Recombinant Antibody (VS-0224-XY93), PE
CAT#: VS-0224-XY93
The product is a rat monoclonal antibody which recognizes mouse transforming growth factor, beta receptor I (Tgfbr1). It was purified by protein A or G affinity chromatography. This product has been determined by Enzyme-linked immunosorbent assay, Western blot and Flow cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56473/985_F7_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2441/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31481/66574_B_9_5.jpg
Normal Tissue
Figure 4 Cerebellum
Cells in granular layer Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Purkinje cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31481/66574_B_8_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31481/66574_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31481/66574_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (RH-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106799-TGFBR1
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Specifications
- Immunogen
- S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Tgfbr1 extracellular domain (Ala21-Glu121).
- Host Species
- Rat
- Type
- Rat IgG2a
- Specificity
- Mouse Tgfbr1
- Species Reactivity
- Mouse
- Applications
- ELISA, Western Blot, Flow Cytometry
- Conjugate
- PE
- Related Disease
- Multiple Self-Healing Squamous Epithelioma; Loeys-Dietz Syndrome 1
Product Property
- Purification
- Protein A or G purified
- Buffer
- A saline solution containing BSA
- Preservative
- Sodium Azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in Flow cytometry (10 µL/106 cells).
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# VS-0224-XY93, RRID: AB_3714375)
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Related Diseases
Pancreatic Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Recommended Dilution Buffer
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Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4032z | Mouse Anti-TGFBR1 Recombinant Antibody (clone 43H12) | WB, ELISA, IHC | Mouse IgG1 |
| MOB-2021CT | Recombinant Mouse anti-Human TGFBR1 Monoclonal antibody (9B22) | IHC-P | |
| ZG-0114C | Mouse Anti-TGFBR1 Recombinant Antibody (clone ABT-TGFR1) | IHC-P, IF | Mouse IgG |
| ZG-0115C | Mouse Anti-TGFBR1 Recombinant Antibody (clone ABT-TGFR2) | IHC-P, IF | Mouse IgG |
| VS3-CJ919 | Mouse Anti-TGFBR1 Recombinant Antibody (VS3-CJ919) | IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L077E | TGFα-PE immunotoxin | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC39 | Recombinant Anti-Tgfbr1 Vesicular Antibody, EV Displayed (VS-0425-YC39) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7216 | Anti-Mouse TGFBR1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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