Anti-TP53 Antibody Prodrug, Protease Activated (VS-1025-YC35)
CAT#: VS-1025-YC35
The Anti-TP53 Antibody Prodrug, Protease Activated (VS-1025-YC35) is an antibody prodrug comprised of a recombinant mouse antibody targeting human TP53, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to TP53 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to TP53 effectively. TP53 is arguably the most critical and frequently mutated tumor suppressor gene in human cancer, often called the "guardian of the genome." Its protein, p53, monitors for DNA damage and, if damage is irreparable, triggers cell cycle arrest or apoptosis. Loss of TP53 function-found in over 50% of all human tumors-removes this final quality-control checkpoint, allowing mutated cells to survive and proliferate.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg
Normal Tissue
Figure 5 Oral mucosa
Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg
Normal Tissue
Figure 6 Colon
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53
❮
❯
Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human TP53
- Target
- TP53
- Species Reactivity
- Human
- Isotype
- IgG2a
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.
- Alternative Names
- TP53; Tumor protein p53; P53; BCC7; LFS1; TRP53
- Gene ID
- 7157
- UniProt ID
- P04637
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Related Diseases
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Downloadable Resources
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "TP53"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-994 | Recombinant anti-TP53 antibody svFv Fragment | ELISA, Agonist | scFv |
| HPAB-0001-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); scFv Fragment | IHC, WB, ELISA | Mouse scFv |
| HPAB-0002-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 240); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0003-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 246); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0004-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 421); scFv Fragment | IP, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-072 | Recombinant Anti-p53 VHH Single Domain Antibody (PNBL-072) | Inhib, IP, WB, ELISA, FuncS | Llama VHH |
| HPAB-0746-YJ-VHH | Camelid Anti-TP53 Recombinant Single Domain Antibody (HPAB-0746-YJ-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1423CT | Recombinant Mouse anti-Human TP53 Monoclonal antibody (5E2) | ELISA, FC, ICC, IF, IHC-Fr, IHC-P, IP, WB | |
| HPAB-0001-YC | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85) | IHC, WB, ELISA | Mouse IgG2a |
| HPAB-0002-YC | Mouse Anti-TP53 Recombinant Antibody (clone 240) | IP, ELISA | Mouse IgG1, κ |
| HPAB-0003-YC | Mouse Anti-TP53 Recombinant Antibody (clone 246) | IP, ELISA | Mouse IgG1, κ |
| HPAB-0004-YC | Mouse Anti-TP53 Recombinant Antibody (clone 421) | IP, ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3644 | Rabbit Anti-TP53 Recombinant Antibody (clone DS3644AB) | IHC-P | Rabbit IgG |
| MOR-4524 | Rabbit Anti-TP53 Recombinant Antibody (clone TH32DS) | WB, IF, ICC | Rabbit IgG |
| MOR-4698 | Rabbit Anti-TP53 Recombinant Antibody (clone TH212DS) | WB, IF, ICC, FC, ChIP, FRET | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MD-ZBF-YC001 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-1.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC002 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-2.1) | WB, ICC, IHC | Mouse antibody |
| MD-ZBF-YC003 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-3.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC004 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-4.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC005 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-5.1) | WB, ICC, IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment | IHC, WB, ELISA | Mouse Fab |
| HPAB-0002-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0003-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 246); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0004-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 421); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0005-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone ME1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0633CQ | Mouse Anti-TP53 Recombinant Antibody (clone p53-11) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY261 | AbPlus™ Anti-TP53 Magnetic Beads (240) | IP, Protein Purification | |
| VS-0724-YC1290 | AbPlus™ Anti-TP53 Magnetic Beads (VS-0724-YC1290) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY361 | Mouse Anti-NHP TP53 Recombinant Antibody (clone BP53-12) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC186 | Recombinant Anti-TP53 (AA 213-218 x AA 370-378) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC349 | SmartAb™ Recombinant Anti-TP53 pH-dependent Antibody (Clone 70237-85) | IHC, WB, ELISA | Mouse IgG2a |
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