Rabbit Anti-TP53 Recombinant Antibody (clone TH212DS) (CAT#: MOR-4698)
Recombinant Rabbit anti-TP53 monoclonal antibody for WB, IF, ICC, FCM, ChIP, FRET, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Host: Rabbit
Isotype: Rabbit IgG
Applications: WB, IF, ICC, FC, ChIP, FRET
Specific Inquiry
Subcellular Location
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
Figure 2 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg
Normal Tissue
Figure 5 Oral mucosa
(Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg
(Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg
Figure 6 Colon
(Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg
(Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000141510-TP53
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000141510-TP53
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53
Specifications
- Immunogen
- A peptide corresponding to amino acids 9-19 of P04637.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TP53
- Species Reactivity
- Human
- Clone
- TH212DS
- Applications
- WB, IF, ICC, FC, ChIP, FRET
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ChIP 1 µl
FC 0.5-5 µg x 10^6 cells
FRET Assay-dependent
ICC 0.5-2 µg/mL
IF 0.5-2 µg/mL
WB 0.1-0.5 µg/mL
Target
- Alternative Names
- Tumor Protein P53; Phosphoprotein P53; Antigen NY-CO-13; P53; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; P53 Tumor Suppressor; Tumor Suppressor P53; Tumor Supressor P53; Tumor Protein 53; TRP53; BCC7; LFS1
- Gene ID
- 7157
- UniProt ID
- P04637
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TP53"
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
| DrMAB-994 | Recombinant anti-TP53 antibody svFv Fragment | ELISA, Agonist | scFv |
| HPAB-0001-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); scFv Fragment | IHC, WB, ELISA | Mouse scFv |
| HPAB-M0287-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 6C4); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0662-YJ-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone Ab421); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1568WJ-S(P) | Mouse Anti-TP53 Recombinant Antibody; scFv Fragment (clone 11D3) | ELISA, WB | Mouse scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
| PNBL-072 | Recombinant Anti-p53 VHH Single Domain Antibody (Nb139) | Inhib, IP, WB, ELISA, FuncS | Llama VHH |
| HPAB-0746-YJ-VHH | Camelid Anti-TP53 Recombinant Single Domain Antibody (CBP71YJ) | ELISA | Camelid VHH |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
| MOR-3644 | Rabbit Anti-TP53 Recombinant Antibody (clone DS3644AB) | IHC-P | Rabbit IgG |
| MOR-4524 | Rabbit Anti-TP53 Recombinant Antibody (clone TH32DS) | WB, IF, ICC | Rabbit IgG |
MHC Tetramer for Cancer
Model Organism Antibody
| CAT | Product Name | Application | Type |
| MD-ZBF-YC001 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-1.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC002 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-2.1) | WB, ICC, IHC | Mouse antibody |
| MD-ZBF-YC003 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-3.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC004 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-4.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC005 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-5.1) | WB, ICC, IHC | Mouse antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
| HPAB-0001-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment | IHC, WB, ELISA | Mouse Fab |
| HPAB-0002-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-M0287-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 6C4); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0662-YJ-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone Ab421); Fab Fragment | ELISA | Mouse Fab |
| HPAB-1568WJ-F(E) | Mouse Anti-TP53 Recombinant Antibody; Fab Fragment (clone 11D3) | ELISA, WB | Mouse Fab |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
| EPAF-0633CQ | Mouse Anti-TP53 Recombinant Antibody (clone p53-11) | WB | Mouse IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
| MRO-2103-CN | Rabbit Anti-TP53 Polyclonal Antibody (MRO-2103-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2400-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-683) | WB, IF, IHC | Rabbit IgG |
| MRO-2401-CN | Rabbit Anti-TP53 Recombinant Antibody (clone CBACN-684) | WB, IF, IP | Rabbit IgG |
| FAMAB-1279CQ | Mouse Anti-TP53 Recombinant Antibody (clone PAb421) | WB, IF, IB | Mouse IgG2a |
| ZG-0190U | Mouse Anti-TP53 Recombinant Antibody (clone 6H5E7) | ELISA, WB, IHC | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot
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