Is this antibody specific to a certain epitope?

Yes, the clone p53-11 is an epitope-specific mouse antibody that specifically recognizes human TP53. It is designed for use in Western Blot (WB) and other immunological assays for precise detection of the protein.

What is the isotype and format of this product?

This is a Mouse IgG antibody. It is provided as a recombinant monoclonal antibody, ensuring superior lot-to-lot consistency. It is supplied unconjugated with>95% purity as determined by SDS-PAGE.

Mouse Anti-TP53 Recombinant Antibody (clone p53-11)

CAT#: EPAF-0633CQ

This product is a mouse antibody that specifically recognizes TP53 from human. The antibody p53-11 is an epitope-specific antibody that can be used in WB and other immunological assays.

Gene Expression

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A549

Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg

Normal Tissue Figure 5 Oral mucosa

Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg

Normal Tissue Figure 6 Colon

Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

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Specifications

Host Species
Mouse

Type
Mouse IgG

Specificity
Human TP53

Species Reactivity
Human

Clone
p53-11

Applications
WB

Conjugate
Unconjugated

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
TP53; Cellular tumor antigen p53; Human TP53; Human

Gene ID
7157

UniProt ID
P04637

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Breast cancer biomarkers at key points during disease progression

Specific Epitope Recognition

The p53-11 clone is fantastic for specifically recognizing human TP53. As an epitope-specific antibody, it provides very clean results in Western Blot assays, which is essential for our detailed studies on p53 isoforms and mutations in cancer cells.

Dependable Recombinant Mouse Antibody

We prefer this recombinant mouse antibody for its consistency. It has been a reliable reagent in our lab for monitoring TP53 expression under various stress conditions. The antibody is stable and easy to use with standard secondary reagents.

Q&As

Is this antibody specific to a certain epitope?

A: Yes, the clone p53-11 is an epitope-specific mouse antibody that specifically recognizes human TP53. It is designed for use in Western Blot (WB) and other immunological assays for precise detection of the protein.
What is the isotype and format of this product?

A: This is a Mouse IgG antibody. It is provided as a recombinant monoclonal antibody, ensuring superior lot-to-lot consistency. It is supplied unconjugated with>95% purity as determined by SDS-PAGE.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - EPAF-0633CQ.pdf

MSDS

MSDS - EPAF-0633CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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