Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-5.1)
CAT#: MD-ZBF-YC005
This product is a mouse monoclonal antibody that specifically targeting tp53 from zebrafish, which is a model organism for the study of human disease.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg
Normal Tissue
Figure 5 Oral mucosa
Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg
Normal Tissue
Figure 6 Colon
Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53
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Specifications
- Immunogen
- His10-p53 fusion protein
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse antibody
- Specificity
- Zebrafish tp53
- Species Reactivity
- Zebrafish
- Clone
- ZFp53-5.1
- Applications
- Western Blot, Immunocytochemistry, Immunohistochemistry
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Customer Review
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Excellent Specificity
I've been using the Anti-tp53 Antibody from Creative Biolabs for my research on TP53 mutations. The antibody shows excellent specificity for mutant TP53 proteins, which has greatly enhanced the accuracy of my Western blot analyses. The clear and sharp bands made data interpretation straightforward. It's reassuring to have a reliable antibody that consistently performs well across different experiments.
Reliable for IHC
We utilized the Anti-tp53 Antibody for immunohistochemistry on FFPE tissue samples. The antibody produced strong and specific staining with minimal background. The antigen retrieval process was easy to optimize, and the results have been reproducible. This antibody has become a staple in our lab for studying TP53 expression in tissue specimens.
Q&As
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What applications can the Anti-tp53 Antibody be used for in research?
A: The Anti-tp53 Antibody is versatile and can be used in various research applications, including Western blotting, immunohistochemistry, and flow cytometry. It is especially useful for detecting mutant forms of the TP53 protein. This antibody helps researchers study TP53's role in cell cycle regulation, apoptosis, and its implications in cancer research.
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What kind of support does Creative Biolabs offer for troubleshooting if I encounter issues with the Anti-tp53 Antibody?
A: Creative Biolabs offers comprehensive technical support for their products. If you encounter any issues with the Anti-tp53 Antibody, you can contact their technical support team for assistance. They can provide guidance on protocol optimization, troubleshooting tips, and solutions to common problems to ensure successful outcomes in your experiments.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-072 | Recombinant Anti-p53 VHH Single Domain Antibody (PNBL-072) | Inhib, IP, WB, ELISA, FuncS | Llama VHH |
| HPAB-0746-YJ-VHH | Camelid Anti-TP53 Recombinant Single Domain Antibody (HPAB-0746-YJ-VHH) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3644 | Rabbit Anti-TP53 Recombinant Antibody (clone DS3644AB) | IHC-P | Rabbit IgG |
| MOR-4524 | Rabbit Anti-TP53 Recombinant Antibody (clone TH32DS) | WB, IF, ICC | Rabbit IgG |
| MOR-4698 | Rabbit Anti-TP53 Recombinant Antibody (clone TH212DS) | WB, IF, ICC, FC, ChIP, FRET | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MD-ZBF-YC001 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-1.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC002 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-2.1) | WB, ICC, IHC | Mouse antibody |
| MD-ZBF-YC003 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-3.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC004 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-4.1) | WB, ICC | Mouse antibody |
| MD-ZBF-YC006 | Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-6.1) | WB, ICC, IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); scFv Fragment | IHC, WB, ELISA | Mouse scFv |
| HPAB-0002-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 240); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0003-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 246); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0004-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone 421); scFv Fragment | IP, ELISA | Mouse scFv |
| HPAB-0005-YC-S(P) | Mouse Anti-TP53 Recombinant Antibody (clone ME1); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0001-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment | IHC, WB, ELISA | Mouse Fab |
| HPAB-0002-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0003-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 246); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0004-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone 421); Fab Fragment | IP, ELISA | Mouse Fab |
| HPAB-0005-YC-F(E) | Mouse Anti-TP53 Recombinant Antibody (clone ME1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0633CQ | Mouse Anti-TP53 Recombinant Antibody (clone p53-11) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0157F | Mouse Anti-TP53 Recombinant Antibody (MOB-0157F) | IHC-P, IF | Mouse IgG |
| MOB-0159F | Mouse Anti-TP53 Recombinant Antibody (MOB-0159F) | IHC-P, IF, WB | Mouse IgG |
| ZG-0190U | Mouse Anti-TP53 Recombinant Antibody (clone 6H5E7) | ELISA, WB, IHC | Mouse IgG |
| VS4-CJ139 | Recombinant Mouse Anti-TP53 Antibody (VS4-CJ139) | ELISA, WB, IHC, IF | Mouse IgG |
| VS3-FY1472 | Recombinant Rabbit Anti-Tp53 Antibody (clone R01-2B9) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY261 | AbPlus™ Anti-TP53 Magnetic Beads (240) | IP, Protein Purification | |
| VS-0724-YC1290 | AbPlus™ Anti-TP53 Magnetic Beads (VS-0724-YC1290) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY361 | Mouse Anti-NHP TP53 Recombinant Antibody (clone BP53-12) | WB, IHC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC186 | Recombinant Anti-TP53 (AA 213-218 x AA 370-378) Biparatopic Antibody, Tandem scFv | ELISA | Tandem scFv |
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