Recombinant anti-TP53 antibody svFv Fragment (CAT#: DrMAB-994)

Recombinant Antibody binds selectively to TP53.


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ELISA

Figure 1 ELISA in vitro binding of scFvs to immobilized full length but not C-terminus truncated p53 (p53D320-393).

Figure 1 ELISA in vitro binding of scFvs to immobilized full length but not C-terminus truncated p53 (p53D320-393).

Purified mAbs PAb421 or 11D3 or periplasmic extracts containing scFvs 421 and 11D3 were incubated with either recombinant human wt p53 purified from E. coli extract.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene, 18(2), 551-557.

ELISA

Figure 2 scFvs binds to immobilized full length but not C-terminus truncated p53.

Figure 2 scFvs binds to immobilized full length but not C-terminus truncated p53.

Purified mAbs PAb421 or 11D3 or periplasmic extracts containing scFvs 421 and 11D3 were incubated with either recombinant human wt p53 purified from cytosolic extract from baculovirus-infected insect cells expressing p53D320 -393.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene, 18(2), 551-557.

Agonist

Figure 3 The scFvs enhance the DNA binding ability of p53 in vitro.

Figure 3 The scFvs enhance the DNA binding ability of p53 in vitro.

Periplasmic extract of scFv421 was incubated with purified wild-type or mutant p53 His273, and DNA binding determined by electrophoretic mobility shift assay.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments.ncogene, 18(2), 551-557.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Type
  • scFv
  • Species Reactivity
  • Human
  • Applications
  • ELISA, Agonist

Applications

  • Application Notes
  • The TP53 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Agonist.

Target

  • Alternative Names
  • TP53; tumor protein p53; P53; BCC7; LFS1; TRP53; cellular tumor antigen p53; antigen NY-CO-13; mutant tumor protein 53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; tumor protein 53

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT Product Name Application Type
EPAF-0633CQ Mouse Anti-TP53 Recombinant Antibody (clone p53-11) WB Mouse IgG

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