Recombinant anti-TP53 antibody svFv Fragment (CAT#: DrMAB-994)

Recombinant Antibody binds selectively to TP53.

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ELISA

Figure 1 ELISA in vitro binding of scFvs to immobilized full length but not C-terminus truncated p53 (p53D320-393).

Purified mAbs PAb421 or 11D3 or periplasmic extracts containing scFvs 421 and 11D3 were incubated with either recombinant human wt p53 purified from E. coli extract.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene, 18(2), 551-557.

ELISA

Figure 2 scFvs binds to immobilized full length but not C-terminus truncated p53.

Purified mAbs PAb421 or 11D3 or periplasmic extracts containing scFvs 421 and 11D3 were incubated with either recombinant human wt p53 purified from cytosolic extract from baculovirus-infected insect cells expressing p53D320 -393.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene, 18(2), 551-557.

Agonist

Figure 3 The scFvs enhance the DNA binding ability of p53 in vitro.

Periplasmic extract of scFv421 was incubated with purified wild-type or mutant p53 His273, and DNA binding determined by electrophoretic mobility shift assay.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments.ncogene, 18(2), 551-557.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Type
  • scFv
  • Species Reactivity
  • Human
  • Applications
  • ELISA, Agonist

Applications

  • Application Notes
  • The TP53 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Agonist.

Target

  • Alternative Names
  • TP53; tumor protein p53; P53; BCC7; LFS1; TRP53; cellular tumor antigen p53; antigen NY-CO-13; mutant tumor protein 53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; tumor protein 53

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Related Resources

  • Related Diseases

Downloads

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Single-domain Antibody

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MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC036 A*02:01/Human p53 (GLAPPQHLIRV) MHC Tetramer FCM
MHC-LC037 A*02:01/Human p53 (GLAPPQHLIRV) MHC Tetramer FCM
MHC-LC038 A*02:01/Human p53 (GLAPPQHLIRV) MHC Tetramer FCM
MHC-LC051 A*02:01/Human p53 (KLCPVQLWV) MHC Tetramer FCM
MHC-LC052 A*02:01/Human p53 (KLCPVQLWV) MHC Tetramer FCM

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3644 Rabbit Anti-TP53 Recombinant Antibody (clone DS3644AB) IHC-P Rabbit IgG
MOR-4524 Rabbit Anti-TP53 Recombinant Antibody (clone TH32DS) WB, IF, ICC Rabbit IgG
MOR-4698 Rabbit Anti-TP53 Recombinant Antibody (clone TH212DS) WB, IF, ICC, FC, ChIP, FRET Rabbit IgG

Model Organism Antibody

CAT Product Name Application Type
MD-ZBF-YC001 Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-1.1) WB, ICC Mouse antibody
MD-ZBF-YC002 Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-2.1) WB, ICC, IHC Mouse antibody
MD-ZBF-YC003 Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-3.1) WB, ICC Mouse antibody
MD-ZBF-YC004 Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-4.1) WB, ICC Mouse antibody
MD-ZBF-YC005 Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-5.1) WB, ICC, IHC Mouse antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0001-YC-S(P) Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); scFv Fragment IHC, WB, ELISA Mouse scFv
HPAB-0002-YC-S(P) Mouse Anti-TP53 Recombinant Antibody (clone 240); scFv Fragment IP, ELISA Mouse scFv
HPAB-0003-YC-S(P) Mouse Anti-TP53 Recombinant Antibody (clone 246); scFv Fragment IP, ELISA Mouse scFv
HPAB-0004-YC-S(P) Mouse Anti-TP53 Recombinant Antibody (clone 421); scFv Fragment IP, ELISA Mouse scFv
HPAB-0662-YJ-S(P) Mouse Anti-TP53 Recombinant Antibody (clone Ab421); scFv Fragment ELISA Mouse scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0001-YC-F(E) Mouse Anti-TP53 Recombinant Antibody (clone 70237-85); Fab Fragment IHC, WB, ELISA Mouse Fab
HPAB-0002-YC-F(E) Mouse Anti-TP53 Recombinant Antibody (clone 240); Fab Fragment IP, ELISA Mouse Fab
HPAB-0003-YC-F(E) Mouse Anti-TP53 Recombinant Antibody (clone 246); Fab Fragment IP, ELISA Mouse Fab
HPAB-0004-YC-F(E) Mouse Anti-TP53 Recombinant Antibody (clone 421); Fab Fragment IP, ELISA Mouse Fab
HPAB-0005-YC-F(E) Mouse Anti-TP53 Recombinant Antibody (clone ME1); Fab Fragment ELISA Mouse Fab

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0633CQ Mouse Anti-TP53 Recombinant Antibody (clone p53-11) WB Mouse IgG

Recombinant Antibody

CAT Product Name Application Type
ZG-0344C Mouse Anti-TP53 Recombinant Antibody (ZG-0344C) WB Mouse IgG
ZG-0345C Mouse Anti-TP53 Recombinant Antibody (ZG-0345C) WB, IHC, ELISA Mouse IgG
ZG-0346C Mouse Anti-TP53 Recombinant Antibody (ZG-0346C) WB Mouse IgG
ZG-0347C Mouse Anti-TP53 Recombinant Antibody (ZG-0347C) WB Mouse IgG
ZG-0767F Mouse Anti-TP53 Recombinant Antibody (clone BP53-12) WB, IP, IHC-P Mouse IgG2a

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For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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