Recombinant anti-TP53 antibody svFv Fragment

Figure 1 ELISA in vitro binding of scFvs to immobilized full length but not C-terminus truncated p53 (p53D320-393).

Purified mAbs PAb421 or 11D3 or periplasmic extracts containing scFvs 421 and 11D3 were incubated with either recombinant human wt p53 purified from E. coli extract.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene, 18(2), 551-557.

Figure 2 scFvs binds to immobilized full length but not C-terminus truncated p53.

Purified mAbs PAb421 or 11D3 or periplasmic extracts containing scFvs 421 and 11D3 were incubated with either recombinant human wt p53 purified from cytosolic extract from baculovirus-infected insect cells expressing p53D320 -393.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments. Oncogene, 18(2), 551-557.

Figure 3 The scFvs enhance the DNA binding ability of p53 in vitro.

Periplasmic extract of scFv421 was incubated with purified wild-type or mutant p53 His273, and DNA binding determined by electrophoretic mobility shift assay.

de Fromentel, C. C., Gruel, N., Venot, C., Debussche, L., Conseiller, E., Dureuil, C., ... & Bracco, L. (1999). Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti-p53 single chain Fv fragments.ncogene, 18(2), 551-557.