Human Anti-VEGFA Recombinant Antibody (clone MIL60)
CAT#: HPAB-0169-WJ
The recombinant antibody is capable of very effectively inhibiting the growth of tumor cells A431 and HUVEC and has a very remarkable inhibition effect, and the inhibition rate of the recombinant antibody tothe tumor cell HUVEC is nearly 4 times of that of Bevacizumab.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGF
- Species Reactivity
- Human
- Clone
- MIL60
- Applications
- ELISA, Neut, Inhib, IHC, WB
- Related Disease
- Cancers
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The VEGF antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Neutralization, Inhibition, Immunohistochemistry, Western Blot.
For ELISA: ELISA plates were coated with 0.5 µg/ml VEGF fusion protein at 4 °C overnight and then blocked with 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) containing 0.05% Tween-20 for one hour at 37 °C. Diluted MIL60 or Bevacizumab were added as the primary antibody and incubated for 2 h at 37 °C. After washing, HRP-conjugated goat anti-human IgG was incubated for one hour at room temperature. Binding signals were visualized using o-phenylenediamine dihydrochloride substrate and the light absorbance was measured using an ELISA reader at 492 nm. For competition ELISA, MIL60, Bevacizumab or a negative control antibody (IgG) were individually mixed at increasing concentrations with an equal volume of the competitors. The antibody mixtures were added as the primary antibodies and all experiments were performed in triplicate.
For IHC: One week after the last treatment, the tumors of the mice were removed and fixed with 10% formaldehyde. Paraffin-embedded tissue sections were processed, deparaffinized, rehydrated and quenched of endogenous peroxidase activity. Sections were first stained with anti-CD31 antibody (dilution 1∶100) and then incubated with HRP-conjugated secondary antibody. Finally, the sections were developed with diaminobenzidine and counterstained with hematoxylin. Pictures were captured using an OLYMPUS BX5 microscope and an UPlanFL N digital camera (10×0.13 numeric aperture objective). Any single, brown-stained cell or cluster of EC that was clearly separated from adjacent microvessels, tumor cells and other connective tissue elements was considered a vessel. The number of CD31-position capillaries was counted from five randomly chosen fields.
For Western Blot: HUVECs were seeded in six-well plates at a density of 8×10⁵ cells/ml. After being serum-starved overnight, they were cultivated in serum-free ECM that was supplemented with 50 ng/ml VEGF and different concentrations of MIL60 or Bevacizumab for 30 min. Thereafter, the supernatant of the cell lysate was subjected to SDS–PAGE analysis using a 12% gel and transferred onto a nitrocellulose membrane. The membrane was blocked with non-fat 5% milk for 1 h at room temperature and then incubated with various antibodies (dilution 1∶1000) overnight at 4 °C. After washing, the membranes were incubated with HRP-conjugated antibodies for an hour at room temperature, and the signals of the membrane were detected and visualized using an enhanced chemiluminescence detection system and autoradiography.
Target
- Alternative Names
- Vascular endothelial growth factor; VEGF
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H73 | Anti-Human VEGFA Recombinant Antibody (TAB-H73) | WB, FC, IP, ELISA, Neut, FuncS, IF | VH - C-kappa - CH2 - CH3 _ V-lambda - CH1 _ H-GAMMA-1 _ L-kappa |
| TAB-011 | Anti-Human VEGF Recombinant Antibody (Bevacizumab) | FC, IP, ELISA, Neut, FuncS, IF, ICC | IgG1 - kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-307CQ-F(E)) | ELISA, Neut | Humanized Fab |
| TAB-009ML | Anti-Human VEGFA Recombinant Antibody scFv Fragment (TAB-009ML) | ELISA, IHC, FC, IP, IF, Inhib | scFv, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L043L | anti-VEGFA immunotoxin Ng76 (scFv)-Luffin-β | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-131 | Human Anti-VEGFA Recombinant Antibody (clone bH1) | Inhib | Human IgG |
| PABW-131 | Human Anti-VEGFA Recombinant Antibody (clone YC159) | WB, ELISA | Human IgG |
| HPAB-0114-WJ | Human Anti-VEGFA Recombinant Antibody (clone FD006) | ELISA, IHC, WB | Humanized IgG |
| HPAB-0152-WJ | Human Anti-VEGFA Recombinant Antibody (HPAB-0152-WJ) | ELISA | Human IgG1 |
| HPAB-S0147-YC | Human Anti-VEGFA Recombinant Antibody (clone A4.6.1 hu2.10V) | ELISA, FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-131 | Human Anti-VEGFA Recombinant Antibody (clone bH1); Fab Fragment | WB, IF, FuncS | Human Fab |
| PFBW-131 | Human Anti-VEGFA Recombinant Antibody (clone YC159); Fab Fragment | WB, ELISA | Human Fab |
| HPAB-0715-FY-S(P) | Mouse Anti-VEGFA Recombinant Antibody (clone AB014); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0950-FY-S(P) | Camelid Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0950-FY-S(P)) | Inhib | Camelid scFv |
| HPAB-J0270-YC-F(E) | Human Anti-VEGFA Recombinant Antibody (clone XPA.10.064.03); Fab Fragment | ELISA, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-284CQ | Human Anti-VEGFA Recombinant Antibody (TAB-284CQ) | WB | Human antibody |
| TAB-284CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-284CQ-F(E)) | ELISA | Human Fab |
| TAB-304CQ-F(E) | Anti-Human VEGFA Recombinant Antibody Fab Fragment (2H1) | ELISA, WB | Human antibody |
| TAB-305CQ-F(E) | Anti-Human VEGFA Recombinant Antibody Fab Fragment (2H1-32) | ELISA, WB | Human antibody |
| TAB-465CQ | Anti-Human VEGFA Recombinant Antibody (TAB-465CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-306CQ | Mouse Anti-VEGFA Recombinant Antibody (TAB-306CQ) | FuncS, Inhib | Mouse IgG |
| TAB-306CQ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-306CQ-S(P)) | ELISA, Neut | Mouse scFv |
| TAB-306CQ-F(E) | Mouse Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-306CQ-F(E)) | ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0623MZ | Chicken Anti-VEGF Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2258CQ | Mouse Anti-VEGFA Recombinant Antibody (clone 4B2-8) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-2260CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL737) | WB, ELISA, Neut | Mouse IgG2b |
| NEUT-2261CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL738) | WB, IHC, ELISA, Neut | Mouse IgG2b |
| NEUT-2262CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL928) | Neut | Mouse IgG1, κ |
| NEUT-2263CQ | Mouse Anti-VEGFA Recombinant Antibody (clone CBL984) | ELISA, Neut, WB | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2259CQ | Mouse Anti-VEGFA Recombinant Antibody (clone A.4.6.1) | ELISA, IP, WB, Block, FC, IHC | Mouse IgG1, κ |
| NEUT-2267CQ | Mouse Anti-VEGFA Recombinant Antibody (NEUT-2267CQ) | WB, Block | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3835 | Rabbit Anti-VEGFA Recombinant Antibody (clone DS3835AB) | FC, ICC, IF, IHC-P, IP, WB | Rabbit IgG |
| MOR-4758 | Rabbit Anti-Vegfa Recombinant Antibody (clone TH272DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MD-ZBF-YC024 | Camelid Anti-VEGFA Recombinant Single Domain Antibody (MD-ZBF-YC024) | ELlSA | Camelid VHH |
| HPAB-N0310-YC-VHNAR | Recombinant Shark Anti-VEGFA Single Domain Antibody (HPAB-N0310-YC-VHNAR) | ELISA, FC, WB | Shark vNAR |
| HPAB-N0311-YC-VHNAR | Recombinant Shark Anti-VEGFA Single Domain Antibody (HPAB-N0311-YC-VHNAR) | ELISA, FC, WB | Shark vNAR |
| HPAB-0337-YJ-vNAR | Recombinant Shark Anti-VEGFA vNAR (HPAB-0337-YJ-vNAR) | FC, ELISA, Inhib, Neut | Shark vNAR |
| NBD-0090-YJ-VHH | Camelid Anti-VEGFA Recombinant Single Domain Antibody (NBD-0090-YJ-VHH) | ELISA, WB, Inhib | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0151-FY | Human Anti-VEGFA Recombinant Antibody (clone rhVVH) | WB, ELISA, Inhib | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-465CQ | Afuco™ Anti-VEGFA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-465CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-011 | Afuco™ Anti-VEGFA ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-011) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0169-WJ-S(P) | Human Anti-VEGFA Recombinant Antibody (clone MIL60); scFv Fragment | ELISA | Human scFv |
| HPAB-0512-WJ-S(P) | Human Anti-VEGFA Recombinant Antibody (clone F6); scFv Fragment | ELISA | Human scFv |
| HPAB-0821-WJ-S(P) | Mouse Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0821-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-0938WJ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (HPAB-0938WJ-S(P)) | ELISA | Human scFv |
| HPAB-J0270-YC-S(P) | Human Anti-VEGFA Recombinant Antibody (clone XPA.10.064.03); scFv Fragment | ELISA, Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY273 | AbPlus™ Anti-VEGFA Magnetic Beads (A.4.6.1) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY540 | Mouse Anti-NHP VEGFA Recombinant Antibody (clone 26503) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY46 | Human Anti-VEGFA (clone G6-31) scFv-Fc Chimera | Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC709 | Recombinant Anti-VEGFA Vesicular Antibody, EV Displayed (VS-0425-YC709) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC190 | Recombinant Anti-VEGFA (N-terminus x C-terminus) Biparatopic Antibody, Tandem scFv (Clone C1039 x Clone c870) | ELISA | Tandem scFv |
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