Mouse Anti-VIM Recombinant Antibody (VS3-CJ514) (CAT#: VS3-CJ514)

• Immunogen
• Recombinant protein corresponding to human Vimentin

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse, Rat VIM

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, IHC

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200), Immunohistochemistry (1:200-1:500).

• Alternative Names
• Vimentin

• Gene ID
• 7431

• UniProt ID
• P08670

• Sequence Similarities
• Belongs to the intermediate filament family.

• Cellular Localization
• Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane, Nucleus

• Post Translation Modifications
• Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
  One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
  Phosphorylated by STK33 (PubMed:18811945).
  Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
  O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
  S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

• Protein Refseq
• NP_003371.2

• Function
• Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
  Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

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