Human Anti-VWF Recombinant Antibody (clone h82D6A3) (CAT#: FAMAB-0297YC)
Provided is humanized antibody, h82D6A3, by variable domain resurfacing and grafting on the constant regions of a human IgG4. The h82D6A3 still binding with high affinity to VWF and retained the inhibitory properties of the murine 82D6A3.
Specific Inquiry
Inhib
Figure 1 Inhibition of VWF-binding to human collagen type III.
Before addition to collagen coated wells, a constant concentration of purified human VWF (250 ng/ml) was pre-incubated with different concentrations of purified h82D6A3 or m82D6A3. Bound VWF was detected with anti-VWF-HRP antibodies. The absorbance of VWF-binding in the absence of 82D6A3 was arbitrarily set as 0% inhibition.
Staelens, S., Desmet, J., Ngo, T. H., Vauterin, S., Pareyn, I., Barbeaux, P., ... & Vanhoorelbeke, K. (2006). Humanization by variable domain resurfacing and grafting on a human IgG4, using a new approach for determination of non-human like surface accessible framework residues based on homology modelling of variable domains. Molecular immunology, 43(8), 1243-1257.
Specifications
- Immunogen
- Human VWF
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG4
- Specificity
- Human VWF
- Species Reactivity
- Human
- Clone
- h82D6A3
- Applications
- Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibion. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone h82D6A3"
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment | Block | Mouse Fab |
| PFBC-133 | Mouse Anti-VWF Recombinant Antibody (clone RU5); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
| HPAB-M0250-YC-F(E) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); Fab Fragment | Inhib, FuncS | Mouse Fab |
| HPAB-M0251-YC-F(E) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); Fab Fragment | Inhib, FuncS | Chimeric (mouse/human) Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); scFv Fragment | Block | Mouse scFv |
| HPAB-0343-WJ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (HPAB-0343-WJ-S(P)) | ELISA, WB | Mouse scFv |
| HPAB-M0250-YC-S(P) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); scFv Fragment | Inhib, FuncS | Mouse scFv |
| HPAB-M0251-YC-S(P) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); scFv Fragment | Inhib, FuncS | Mouse scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-362CQ | Anti-Human VWF Recombinant Antibody (AJvW-2) | ELISA, BL | |
| TAB-362CQ-S(P) | Anti-Human VWF Recombinant Antibody scFv Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-363CQ-S(P)) | ELISA, WB, Inhib | Mouse scFv |
| TAB-362CQ-F(E) | Anti-Human VWF Recombinant Antibody Fab Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (TAB-363CQ-F(E)) | ELISA, WB, Inhib | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-364CQ | Human Anti-VWF Recombinant Antibody (TAB-364CQ) | Inhib | Humanized antibody |
| TAB-364CQ-S(P) | Human Anti-VWF Recombinant Antibody; scFv Fragment (TAB-364CQ-S(P)) | Inhib | Humanized scFv |
| TAB-364CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-364CQ-F(E)) | Inhib | Humanized Fab |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-365CQ | Human Anti-VWF Recombinant Antibody (TAB-365CQ) | Inhib | Chimeric antibody (mouse/human) |
| TAB-365CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-365CQ-S(P)) | Inhib | Mouse scFv |
| TAB-365CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-365CQ-F(E)) | Inhib | Chimeric antibody (mouse/human) |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3860 | Rabbit Anti-VWF Recombinant Antibody (clone DS3860AB) | WB, ICC, IF | Rabbit IgG |
| MOR-0109-FY | Rabbit Anti-VWF Recombinant Antibody (clone AFY0080) | ICC, IHC-P, WB | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0298YC | Mouse Anti-VWF Recombinant Antibody (clone m-82D6A3) | Inhib | Mouse IgG |
| MRO-1590-CN | Rabbit Anti-VWF Recombinant Antibody (clone CBACN-582) | WB, IHC | Rabbit IgG |
| HPAB-0343-WJ | Mouse Anti-VWF Recombinant Antibody (HPAB-0343-WJ) | ELISA, WB | Mouse IgG |
| FAMAB-1291CQ | Human Anti-VWF Recombinant Antibody (clone F8/44/20) | WB, ELISA, IHC | Human IgG1 |
| FAMAB-1292CQ | Human Anti-VWF Recombinant Antibody (clone F8/86) | FACS, ICC, WB, IHC | Human IgG1 |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1628-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1628-FY) | ELISA | Llama VHH |
| HPAB-1629-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1629-FY) | ELISA | Llama VHH |
| HPAB-1630-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1630-FY) | Inhib, ELISA | Llama VHH |
| HPAB-1631-FY | Recombinant Llama Anti-VWF Single Domain Antibody (12A5) | ELISA, SPR, FC | Llama VHH |
| HPAB-1632-FY | Recombinant Llama Anti-VWF Single Domain Antibody (12B4) | Inhib | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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