Rabbit Anti-WT1 Recombinant Antibody (VS3-CJ753) (CAT#: VS3-CJ753)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse WT1

• Species Reactivity
• Human, Mouse

• Applications
• WB, ICC, IF, IHC, FC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.

• Alternative Names
• GUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2

• Gene ID
• 7490

• UniProt ID
• P19544

• Sequence Similarities
• Belongs to the EGR C2H2-type zinc-finger protein family.

• Cellular Localization
• Cytoplasm, Nucleus

• Protein Refseq
• NP_000369.3; NP_001185480.1; NP_001185481.1

• Function
• Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533).
  Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363).
  Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190).
  Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801).
  Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

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