CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383)

Name: CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383)
SKU: VS-0126-XU499
Rating: 5 (1 reviews)

CAT#: VS-0126-XU499

CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383) provides a high-fidelity solution for quantifying murine ICAM-1 expression. The recombinant manufacture of the clone ensures structural uniformity and superior signal clarity in flow cytometry (FC). This tool provides the reliability needed for standardized research into vascular biology and the molecular cues of inflammatory cell migration.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/29_G3_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2142/203_G3_1_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/1937_A10_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line GAMG

Immunofluorescent staining of human cell line GAMG shows localization to plasma membrane, vesicles, nucleoplasm & centrosome.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/2058_A10_4_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_B_9_5.jpg

Normal Tissue Figure 6 Lung

Alveolar cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2126/7230_A_3_4.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_3.jpg

Normal Tissue Figure 8 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_8_5.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4877/13410_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (HHSteC, Karpas-707, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000090339-ICAM1

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Sub Cat	Product Name	Size	Formulation	Conjugate Group	Characteristics	Stability	Size	Conjugation	Endotoxin	Purity	Fc Engineering
VS-0126-XU499-1	CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383), APC-conjugated	25 Tests	Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4.	APC	Protein-based fluorochrome with excitation/emission at ~650/660 nm. Exhibits minimal spectral overlap with FITC and PE, ideal for multicolor panels. High brightness.	Sensitive to light and heat. Store at 4°C in the dark. Avoid repeated freeze-thaw cycles. Stable for ≥1 year under proper storage.
VS-0126-XU499-2	CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383), FITC-conjugated	25 Tests	Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4.	FITC	Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel.	Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh.
VS-0126-XU499-3	CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383), Pacific Blue-conjugated	25 Tests	Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4.	Pacific Blue	Synthetic blue fluorescent dye (Ex/Em: ~410/455 nm). Excellent for violet laser (405 nm) excitation. Low spectral overlap with green/red channel fluorophores, simplifying panel design.	High photostability. Tolerates common fixation protocols well. Store at 4°C in the dark for long-term stability.
VS-0126-XU499-4	CytoStream™ Rat Anti-CD54 Recombinant Antibody (RMC-M1383), PE-conjugated	25 Tests	Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4.	PE	Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation.	Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance.
VS-0126-XU499-5	CytoStream™ Purified Rat Anti-CD54 Recombinant Antibody (RMC-M1383)	100 ug		None	The native antibody without any fluorophore. Used as a primary antibody in indirect staining or for functional assays (blocking, neutralization). Requires a compatible fluorescent secondary antibody for detection.	Typically exhibits the highest intrinsic stability among all formats. Storage conditions are defined by the antibody's own formulation buffer.

More Infomation

Specifications

Host Species
Rat

Type
Monoclonal Antibody

Specificity
CD54

Species Reactivity
Mouse

Applications
FC

Product Property

Clonality
Monoclonal

Storage
Store at 4˚C.

Shipping
Ice Packs

Applications

Application Notes
Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.

Target

Alternative Names
Icam1; Icam-1; Ly-47; MALA-2

Gene ID
15894

UniProt ID
P35331

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Related Diseases

Rheumatoid Arthritis

AGE-RAGE Signaling Pathway in Diabetic Complications

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0126-XU499.pdf

MSDS

MSDS - VS-0126-XU499.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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