Human Anti-RGMA scFv-Fc Chimera (VS-0425-FY173)
CAT#: VS-0425-FY173
Anti-RGMA scFv-FC is a recombinant protein consisting of a single-chain variable fragment (scFv) derived from antibodies targeting the RGMA protein, fused to the Fc portion of an immunoglobulin molecule. This product is designed for research and therapeutic applications involving RGMA (Repulsive Guidance Molecular A), a protein involved in various biological processes, including neuronal guidance and immune regulation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39880/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44222/1038_D3_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12343/32697_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12343/32439_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12343/32439_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12343/32439_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12343/32439_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, BEWO, MOLT-4, NTERA-2, REH)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182175-RGMA
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Specifications
- Immunogen
- Human RGM A as an immunogen.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human RGMA
- Species Reactivity
- Human
- Applications
- ELISA, Inhibition
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- It selectively inhibits binding of RGM A to its receptor Neogenin and to bone morphogenetic proteins 2 and 4 (BMP-2, BMP-4).
It neutralizes the neurite outgrowth inhibit activity of human RGM A.
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Repulsive Guidance Molecule Family Member A; RGM Domain Family, Member A; RGM; Repulsive Guidance Molecule A; RGM Domain Family Member A
- Gene ID
- 56963
- UniProt ID
- Q96B86
- Long Name
- Repulsive Guidance Molecule Family Member A
- Sequence Similarities
- Belongs to the repulsive guidance molecule (RGM) family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.
Glycosylation at Asn114, Asn159, and Asn389
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 4 sites, 2 N-linked glycans (2 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001159755.1; NP_001159758.1; NP_001159759.1; NP_001159760.1; NP_001159761.1
- Function
- Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system.
Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses.
Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition.
Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway.
It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| HPAB-0637-CN-S(P) | Mouse Anti-RGMA Recombinant Antibody; scFv Fragment (HPAB-0637-CN-S(P)) | ELISA, FC, IF | Mouse scFv |
| HPAB-0638-CN-S(P) | Human Anti-RGMA Recombinant Antibody; scFv Fragment (HPAB-0638-CN-S(P)) | ELISA, FC, IF | Humanized scFv |
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| CAT | Product Name | Application | Type |
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| HPAB-0635-CN-F(E) | Rat Anti-RGMA Recombinant Antibody; Fab Fragment (HPAB-0635-CN-F(E)) | ELISA, FC, IF | Rat Fab |
| HPAB-0636-CN-F(E) | Rat Anti-RGMA Recombinant Antibody; Fab Fragment (HPAB-0636-CN-F(E)) | ELISA | Rat Fab |
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| HPAB-0617YY-F(E) | Human Anti-RGMA Recombinant Antibody; Fab Fragment (HPAB-0617YY-F(E)) | ELISA, FuncS | Humanized Fab |
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