Mouse Anti-FLT1 scFv-Fc Chimera (VS-0425-FY53)
CAT#: VS-0425-FY53
The anti-FLT1 scFv-Fc fusion protein is an engineered therapeutic designed to target Fms-like tyrosine kinase 1 (FLT1), a receptor critically involved in angiogenesis. This construct combines a single-chain variable fragment (scFv) to ensure high-affinity binding to FLT1, with an Fc domain to enhance its stability and extend its circulating half-life. 1 By selectively inhibiting FLT1 signaling, this protein aims to suppress pathological angiogenesis, which plays a pivotal role in diseases such as cancer and ocular neovascularization. The fusion to the Fc portion allows for longer lasting effect in the blood stream of treated patients. Research is being conducted to explore the proteins abilities to inhibit angiogenesis, and therefore it's therapeutic capacity.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/90_G3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/1368_A5_2_blue_green.jpg
Normal Tissue
Figure 3 Testis
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11740/142518_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HMC-1, HUVEC TERT2, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102755-FLT1
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Specifications
- Immunogen
- The antibody was obtained by immunizing BALB/c mice with a peptide, A4, which was derived from the sequence of VEGFR-1. This peptide was crucial in eliciting an immune response, leading to the production of specific antibodies targeting the VEGFR-1 receptor.
- Host Species
- Mouse
- Type
- Mouse IgG1, scFv-Fc
- Specificity
- The antibody specifically binds to the extracellular domain of VEGFR-1.
- Species Reactivity
- Human
- Applications
- Western Blot, ELISA, Inhibition
- Related Disease
- Non-small cell lung cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 10 μg/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Fms Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-Related Tyrosine Kinase 1 (Vascular Endothelial Growth Factor/Vascular Permeability Factor Receptor); Vascular Endothelial Growth Factor Receptor 1; Tyrosine-Protein Kinase Receptor FLT; Tyrosine-Protein Kinase FRT; Fms-Like Tyrosine Kinase 1; EC 2.7.10.1;
- Gene ID
- 2321
- UniProt ID
- P17948
- Long Name
- Fms Related Tyrosine Kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Cellular Localization
- Cell membrane; Single-pass type I membrane protein; Endosome
- Post Translation Modifications
- N-glycosylated.
Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation.
Autophosphorylated on tyrosine residues upon ligand binding.
- Protein Refseq
- NP_001153392.1; NP_001153502.1; NP_001153503.1; NP_002010.2
- Function
- Acts as a positive regulator of postnatal retinal hyaloid vessel regression.
May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells.
Can promote endothelial cell proliferation, survival and angiogenesis in adulthood.
Its function in promoting cell proliferation seems to be cell-type specific.
Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro).
Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR.
Modulates KDR signaling by forming heterodimers with KDR.
Ligand binding leads to the activation of several signaling cascades.
Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C.
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Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "FLT1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| TAB-008CQ | Mouse Anti-FLT1 Recombinant Antibody (TAB-008CQ) | ELISA, FC | Mouse IgG |
| TAB-008CQ-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (TAB-008CQ-S(P)) | ELISA, FC | Mouse scFv |
| TAB-008CQ-F(E) | Mouse Anti-FLT1 Recombinant Antibody; Fab Fragment (TAB-008CQ-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009CQ | Human Anti-FLT1 Recombinant Antibody (TAB-009CQ) | ELISA | Chimeric antibody (mouse/human) |
| TAB-009CQ-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (TAB-009CQ-S(P)) | ELISA | Mouse scFv |
| TAB-009CQ-F(E) | Human Anti-FLT1 Recombinant Antibody; Fab Fragment (TAB-009CQ-F(E)) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-845CQ | Mouse Anti-FLT1 Recombinant Antibody (clone MAB0702) | WB, Block | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| MOR-1322 | Hi-Affi™ Rabbit Anti-FLT1 Recombinant Antibody (clone DS1322AB) | WB, IHC-P, ICC, IF, IP, ELISA, IHC-P, IHC-Fr | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0137-YC-F(E) | Human Anti-FLT1 Recombinant Antibody (clone 18F1); Fab Fragment | ELISA, FC, Block, WB | Human Fab |
| HPAB-0785-CN-F(E) | Mouse Anti-FLT1 Recombinant Antibody (clone 1-833-527); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-1606LY-F(E) | Human Anti-FLT1 Recombinant Antibody; Fab Fragment (HPAB-1606LY-F(E)) | ELISA | Human Fab |
| HPAB-1607LY-F(E) | Human Anti-FLT1 Recombinant Antibody; Fab Fragment (HPAB-1607LY-F(E)) | ELISA | Human Fab |
| HPAB-M0695-YC-F(E) | Mouse Anti-FLT1 Recombinant Antibody; Fab Fragment (HPAB-M0695-YC-F(E)) | ELISA, WB, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1606LY | Human Anti-FLT1 Recombinant Antibody (HPAB-1606LY) | ELISA | Human IgG |
| HPAB-1607LY | Human Anti-FLT1 Recombinant Antibody (HPAB-1607LY) | ELISA | Human IgG |
| HPAB-1609LY | Human Anti-FLT1 Recombinant Antibody (HPAB-1609LY) | ELISA | Human IgG |
| HPAB-1610LY | Human Anti-FLT1 Recombinant Antibody (HPAB-1610LY) | ELISA | Human IgG |
| HPAB-1611LY | Human Anti-FLT1 Recombinant Antibody (HPAB-1611LY) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-177 | Afuco™ Anti-FLT1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-177) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0673 | PE-A*02:01/Human FLT1 (TLFWLLTL) MHC Tetramer | FCM, IHC | |
| MHC-CN0674 | APC-A*02:01/Human FLT1 (TLFWLLTL) MHC Tetramer | FCM, IHC | |
| MHC-CN0675 | FITC-A*02:01/Human FLT1 (TLFWLLTL) MHC Tetramer | FCM, IHC | |
| MHC-CN0676 | A*02:01/Human FLT1 (TLFWLLTL) MHC Tetramer | FCM, IHC | |
| MHC-CN1412 | PE-A*24:02/Human FLT1 (SYGVLLWEI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0499-YJ-S(P) | Human Anti-FLT1 Recombinant Antibody; scFv Fragment (HPAB-0499-YJ-S(P)) | ELISA, Block, FuncS | Human scFv |
| HPAB-0500-YJ-S(P) | Human Anti-FLT1 Recombinant Antibody; scFv Fragment (HPAB-0500-YJ-S(P)) | ELISA, Block, FuncS | Human scFv |
| HPAB-0501-YJ-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (HPAB-0501-YJ-S(P)) | ELISA, Block, FuncS | Mouse scFv |
| HPAB-1699-FY-F(E) | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZM-01941); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1700-FY-F(E) | Mouse Anti-FLT1 Recombinant Antibody (clone CA0071-Z2ZA-01171); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY850 | Anti-FLT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2581 | Anti-Mouse FLT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2582 | Anti-Zebrafish FLT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2580 | Anti-Human FLT1 Immunohistochemistry Kit | IHC |
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| VS-0425-YC224 | Recombinant Anti-FLT1 Vesicular Antibody, EV Displayed (VS-0425-YC224) | ELISA, FC, Neut, Cell-uptake |
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