Anti-Human VEGFR-1 Recombinant Antibody (TAB-177) (CAT#: TAB-177)
Recombinant monoclonal antibody to VEGFR-1. It is a human monoclonal antibody designed for the treatment of solid tumors.
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ELISA
Figure 1 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in ELISA
ELISA analysis of TAB-177 was performed by coating with human VEGFR-1 protein (His tag). Then blocked with BSA and incubated with TAB-177. A HRP-conjugated goat anti-human IgG was used as the secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in WB
Western blot analysis of TAB-177 was performed by loading human VEGFR-1 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-177 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-VEGFR-1 Recombinant Antibody (TAB-177) in DB
Dot Blot analysis of TAB-177 was performed by coating with human VEGFR-1 protein (His tag).
TAB-177 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Immunogen
- Recombinant human soluble extracellular Flt-1 Ig-like loop 1 to 5 (sFlt-1(D5)).
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- Suitable for use in Neut, ELISA, IF, IP, FuncS, FC, ICC and most other immunological methods.
- MW
- 146.8 kDa
- Related Disease
- Colon cancers
Product Property
- Purity
- >95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- 4°C. For long term storage, aliquot and store at -20°C. Repeated thawing and freezing must be avoided.
Target
- Alternative Names
- Icrucumab;1024603-92-6;IMC-18F1;IMC18F1;FLT1;fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor);FLT;vascular endothelial growth factor receptor 1;VEGFR1;FLT-1;VEGFR-1;fms-like tyrosine kinase 1;tyrosin
- Gene ID
- 2321
- UniProt ID
- P17948
Related Resources
Please refer to Icrucumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Icrucumab.
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-008CQ | Mouse Anti-FLT1 Recombinant Antibody (TAB-008CQ) | ELISA, FC | Mouse IgG |
| TAB-008CQ-S(P) | Mouse Anti-FLT1 Recombinant Antibody; scFv Fragment (TAB-008CQ-S(P)) | ELISA, FC | Mouse scFv |
| TAB-008CQ-F(E) | Mouse Anti-FLT1 Recombinant Antibody; Fab Fragment (TAB-008CQ-F(E)) | ELISA, FC | Mouse Fab |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-177, RRID: AB_3111850)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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