Mouse Anti-HIF1A Recombinant Antibody (clone 1A3) (CAT#: VS3-XY805)
Clone 1A3 is a mouse antibody, which reacts with human HIF1A protein. This antibody is suitable for HIF1A detection by ELISA, WB, IHC, ICC assays.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Figure 4 Gallbladder
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
Specifications
- Immunogen
- Recombinant fragment of human HIF1A expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HIF1A
- Species Reactivity
- Human
- Clone
- 1A3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor); Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1-Alpha; HIF1-ALPHA; BHLHe78; PASD8; MOP1
- Gene ID
- 3091
- UniProt ID
- Q16665
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1828z | Mouse Anti-HIF1A Recombinant Antibody (clone 6F11) | WB, ELISA, IF | Mouse IgG1 |
| MRO-1928-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1928-CN) | WB, IF, FC | Rabbit IgG |
| MRO-1929-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1929-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX510 | Mouse Anti-HIF1A Recombinant Antibody (clone 5H7) | WB, IP, ICC, IHC | Mouse IgG1 |
| VS3-WK1354 | Mouse Anti-HIF1A Recombinant Antibody (VS3-WK1354) | WB, IHC | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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