Mouse Anti-PRKAA1 Recombinant Antibody (clone B1-E10)
CAT#: VS3-XY1296
Clone B1-E10 is a mouse antibody, which reacts with human PRKAA1 protein. This antibody is suitable for PRKAA1 detection by WB, ICC, IHC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
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Specifications
- Immunogen
- recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PRKAA1
- Species Reactivity
- Human
- Clone
- B1-E10
- Applications
- Immunocytochemistry, Immunohistochemistry, Western Blot
Product Property
- Purification
- Protein A affinity purified
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone B1-E10"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2844 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (DS2844AB) | ELISA, IHC-P, WB | IgG |
| MOR-4672 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (TH186DS) | WB, IF, ICC | IgG |
| MOR-0064-FY | Rabbit Anti-PRKAA1 Recombinant Antibody (clone AFY0035) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0089-CN | Recombinant Mouse Anti-PRKAA1 Monoclonal Antibody (B1-E10) | WB, IF, IHC | Mouse IgG1 |
| MRO-0090-CN | Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (CBACN-025) | WB, IF, IP, FC | Rabbit IgG |
| ZG-0285F | Mouse Anti-PRKAA1 Recombinant Antibody (ZG-0285F) | WB | Mouse IgG |
| VS3-XY3476 | Rabbit Anti-PRKAA1 Recombinant Antibody (clone R02-3H6) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY215 | AbPlus™ Anti-PRKAA1 Magnetic Beads (CBACN-025) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC928 | CytoStream™ Rabbit Anti-PRKAA1 Recombinant Antibody (VS13-YC928) | WB, ICC, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1741 | Anti-PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5671 | Anti-Mouse PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5670 | Anti-Human PRKAA1 Immunohistochemistry Kit | IHC |
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