Mouse Anti-SDC1 Recombinant Antibody (clone 1A3H4)
CAT#: VS3-XY1398
Clone 1A3H4 is a mouse antibody, which reacts with human SDC1 protein. This antibody is suitable for SDC1 detection by ELISA, WB, IHC, FC, ICC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_4_6.jpg
Normal Tissue
Figure 5 Placenta
Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6185/40538_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HBEC3-KT, hTCEpi, U-251 MG, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115884-SDC1
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Specifications
- Immunogen
- Recombinant fragment of human SDC1 (a.a. 28-171) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SDC1
- Species Reactivity
- Human
- Clone
- 1A3H4
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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