Rabbit Anti-CASP3 Polyclonal Antibody (MRO-1720-CN)
CAT#: MRO-1720-CN
This antibody is a rabbit polyclonal antibody that is capable of binding to CASP3.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
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Specifications
- Immunogen
- Peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CASP3 (Caspase 3)
- Species Reactivity
- Human, Mouse
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Peptide affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOR-0475 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone DS475AB) | IF, IHC-Fr, IHC-P, WB | Rabbit IgG |
| MOR-4541 | Hi-Affi™ Rabbit Anti-CASP3 Recombinant Antibody (clone TH50DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| ZG-0016J | Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) | IF, ICC, WB, IHC-P | Mouse IgG |
| ZG-0070J | Mouse Anti-CASP3 Recombinant Antibody (clone 4B8) | IHC-P, IF | Mouse IgG |
| ZG-0071J | Mouse Anti-CASP3 Recombinant Antibody (clone 5H10) | IHC-P, IF | Mouse IgG |
| ZG-0745F | Mouse Anti-CASP3 Recombinant Antibody (clone AM1 4) | WB | Mouse IgG |
| ZG-0124U | Rabbit Anti-CASP3 Recombinant Antibody (clone 6B2) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY41 | AbPlus™ Anti-CASP3 Magnetic Beads (CBACN-011) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY63 | Mouse Anti-NHP CASP3 Recombinant Antibody (VS-1024-XY63) | WB, IHC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC127 | CytoStream™ Rabbit Anti-CASP3 Recombinant Antibody (VS13-YC127) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1001 | Anti-CASP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1002 | Anti-Mouse CASP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1003 | Anti-Porcine CASP3 Immunohistochemistry Kit | IHC |
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