Rabbit Anti-Smad3 (phospho S423/S425) Monoclonal Antibody (RCB-P0281)
CAT#: VS-0126-XL133
Engineered to bind Smad3 only when modified at Serine 423 and 425, this rabbit monoclonal offers superior specificity. It is an ideal laboratory tool for mapping TGF-beta signaling in WB, ICC/IF, and IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46386/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8094/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_7_2_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_8_8.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69409/153288_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166949-SMAD3
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- Smad3 (phospho S423/S425)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; ICC/IF; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- The purification workflow involves a specialized affinity-based adsorption technique to harvest highly refined protein fractions effectively.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Target
- Alternative Names
- MADH3; JV15-2; Mothers against decapentaplegic homolog 3
- UniProt ID
- P84022
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Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Related Diseases
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| VS13-YC1078 | CytoStream™ Rabbit Anti-SMAD3 Recombinant Antibody (VS13-YC1078) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY2106 | Anti-SMAD3 Immunohistochemistry Kit | IHC |
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| VS-0525-XY6718 | Anti-Rat SMAD3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6719 | Anti-Mouse SMAD3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6717 | Anti-Human SMAD3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0126-XL61 | Rabbit Anti-Smad3 (phospho S423/S425) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
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