Rabbit Anti-Syk (phospho Y525) Antibody
CAT#: VS-0126-XL1893
Targeted at the activation loop Tyrosine 525 of Syk. This rabbit polyclonal antibody ensures robust recognition of the active kinase state specifically through validated WB and IHC experimental workflows.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
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Specifications
- Host Species
- Rabbit
- Specificity
- SYK
- Species Reactivity
- Human; Mouse; Rabbit; Monkey
- Applications
- WB; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Strategy-driven elution from affinity columns ensures that this monoclonal antibody maintains its specialized site-recognition capability and structural stability.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
IHC: 1:100-1:300
Target
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Related Signaling Pathways
BCR Signaling Pathway
FcεR1 Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| MOB-937 | Recombinant Anti-human SYK Antibody | WB, ELISA, IHC, FuncS | IgG |
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| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
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| MOB-937-S(P) | Recombinant Anti-human SYK Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| MHH-937 | Recombinant Human Anti-human SYK Antibody | ELISA, WB, Dot, FuncS | IgG |
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| MHH-937-F(E) | Recombinant Human Anti-human SYK Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
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| MHH-937-S(P) | Recombinant Human Anti-human SYK Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
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| CAT | Product Name | Application | Type |
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| HPAB-0225-YC | Rabbit Anti-SYK Recombinant Antibody (HPAB-0225-YC) | IHC, FuncS | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0225-YC-S(P) | Rabbit Anti-SYK Recombinant Antibody; scFv Fragment (HPAB-0225-YC-S(P)) | IHC, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
| CAT | Product Name | Application | Type |
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| VS-0525-XY7041 | Anti-SYK Immunohistochemistry Kit | IHC |
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