Recombinant Mouse Anti-CD14 Antibody (clone 6E10-10G10-4H5) (CAT#: VS3-FY240)
This product is a recombinant mouse antibody that recognizes human CD14 protein. The mAb was purified by affinity chromatography.
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IHC
Figure 1. Recombinant Mouse Anti-CD14 Antibody (clone 6E10-10G10-4H5) in IHC.
Immunohistochemical analysis of paraffin-embedded spleen using CD14 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthesized peptide derived from human CD14
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human CD14
- Species Reactivity
- Human
- Clone
- 6E10-10G10-4H5
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- PBS pH 7.3, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/100-1/500
Target
- Alternative Names
- CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein
- Gene ID
- 929
- UniProt ID
- P08571
- Cellular Localization
- Cell membrane, Golgi apparatus, Membrane, Secreted
- Post Translation Modifications
- N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
- Protein Refseq
- NP_000582.1; NP_001035110.1; NP_001167575.1
- Function
- Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655).
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692).
Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135).
Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211).
Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1583CL | Anti-Human CD14 Recombinant Antibody (18D11) | FC | |
| TAB-1584CL | Anti-Human CD14 Recombinant Antibody (Mil2) | FC, ELISA | |
| TAB-1587CL | Anti-Human CD14 Recombinant Antibody (ZCH-7-2F9) | ELISA, WB | |
| TAB-1583CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (18D11) | FC | |
| TAB-1584CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (Mil2) | FC, ELISA |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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