Recombinant Rabbit Anti-CSF1 Capture Antibody (clone R06-2G2) (CAT#: VS3-WK1748)
This antibody is a rabbit recombinant antibody against human CSF1. It was developed by immunization of rabbit with recombinant human CSF1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R06-2G2 and R05-8L2. The clone R06-2G2 can be used as a capture antibody in ELISA analysis to identify human CSF1.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1709_G12_12_cr5800dfbe06bb5_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1709_G12_12_cr5800dfbe06bb5_selected.jpg
Figure 2 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1876_A10_31_blue_red_green.jpg
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1876_A10_31_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1769_A4_33_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1769_A4_33_blue_red_green.jpg
Figure 4 RNA cell line category: Cell line enhanced (ASC diff, BJ hTERT+, GAMG, HHSteC, HSkMC, hTERT-RPE1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184371-CSF1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184371-CSF1
Specifications
- Immunogen
- Recombinant human CSF1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CSF1
- Species Reactivity
- Human
- Clone
- R06-2G2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Colony Stimulating Factor 1; Colony Stimulating Factor 1 (Macrophage); Macrophage Colony Stimulating Factor 1; Lanimostim; CSF-1; MCSF; Macrophage Colony-Stimulating Factor 1; M-CSF
- Gene ID
- 1435
- UniProt ID
- P09603
- Long Name
- Colony Stimulating Factor 1
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated.
- Protein Refseq
- NP_000748.3
- Function
- Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-245-F(E) | Recombinant Anti-human CSF1 Antibody Fab Fragment | WB, RIA, FuncS | Fab |
| HPAB-0748-WJ-F(E) | Human Anti-CSF1 Recombinant Antibody (clone 88); Fab Fragment | ELISA, WB, FC | Human Fab |
| HPAB-0749-WJ-F(E) | Human Anti-CSF1 Recombinant Antibody (clone 100); Fab Fragment | ELISA, WB, FC | Human Fab |
| HPAB-0750-WJ-F(E) | Human Anti-CSF1 Recombinant Antibody (clone 3.8.3); Fab Fragment | ELISA, WB, FC | Human Fab |
| HPAB-0751-WJ-F(E) | Human Anti-CSF1 Recombinant Antibody (clone 2.7.3); Fab Fragment | ELISA, WB, FC | Human Fab |
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