Recombinant Rabbit Anti-CSF1 Detection Antibody (clone R05-8L2)
CAT#: VS3-WK1749
This antibody is a rabbit recombinant antibody against human CSF1. It was developed by immunization of rabbit with recombinant human CSF1 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R06-2G2 and R05-8L2 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R05-8L2 can be used in ELISA analysis to identify human CSF1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1709_G12_12_cr5800dfbe06bb5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1876_A10_31_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1769_A4_33_blue_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (ASC diff, BJ hTERT+, GAMG, HHSteC, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184371-CSF1
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Specifications
- Immunogen
- Recombinant human CSF1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CSF1
- Species Reactivity
- Human
- Clone
- R05-8L2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Colony Stimulating Factor 1; Colony Stimulating Factor 1 (Macrophage); Macrophage Colony Stimulating Factor 1; Lanimostim; CSF-1; MCSF; Macrophage Colony-Stimulating Factor 1; M-CSF
- Gene ID
- 1435
- UniProt ID
- P09603
- Long Name
- Colony Stimulating Factor 1
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated.
- Protein Refseq
- NP_000748.3
- Function
- Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| HPAB-0532-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0532-CN) | ELISA, FC, Neut | Mouse IgG |
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| HPAB-0534-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0534-CN) | ELISA, FC, Neut | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-501CQ | Mouse Anti-CSF1 Recombinant Antibody (clone 3V17) | Neut, WB | Mouse IgG2 |
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| NEUT-503CQ | Mouse Anti-CSF1 Recombinant Antibody (clone CBL441) | WB, Neut | Mouse IgG2a |
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| CAT | Product Name | Application | Type |
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|---|---|---|---|
| HPAB-0532-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0532-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| HPAB-0533-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0533-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| HPAB-0534-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0534-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| HPAB-0535-CN-F(E) | Human Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0535-CN-F(E)) | ELISA, FC, Neut | Humanized Fab |
| HPAB-0537-CN-F(E) | Human Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0537-CN-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-029ML | Afuco™ Anti-CSF1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-029ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY96 | Human Anti-CSF1 (clone 1.120.1) scFv-Fc Chimera | Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC573 | Recombinant Anti-CSF1 Vesicular Antibody, EV Displayed (VS-0425-YC573) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1704 | Anti-CSF1 Immunohistochemistry Kit | IHC |
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