Recombinant Rabbit Anti-Hnf4A Antibody (clone R01-7D7)
CAT#: VS3-FY691
This product is a recombinant rabbit antibody that recognizes Hnf4A. This antibody has been reported for use in Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation. The clone R01-7D7 is specific for human, mouse, rat Hnf4A. The antigen is mouse hnf-4-alpha recombinant protein.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-Hnf4A Antibody (clone R01-7D7) in WB.
Western Blot analysis of HNF4alpha in K562, C6, Hela lysates using HNF4alpha Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/if_selected.jpg
Normal Tissue
Figure 2 Duodenum
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/13034_A_9_2_val_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_3_1.jpg
Normal Tissue
Figure 4 Colon
Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_8_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101076-HNF4A
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Specifications
- Immunogen
- Mouse HNF-4-alpha recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat Hnf4A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-7D7
- Applications
- Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 53 kDa; Observed MW: 53 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Chromatin Immunoprecipitation: 1/20
Target
- Alternative Names
- Hepatocyte Nuclear Factor 4 Alpha; Nuclear Receptor Subfamily 2 Group A Member 1; Transcription Factor HNF-4; Transcription Factor 14; TCF-14; TCF14; NR2A1; HNF4; Hepatocyte Nuclear Factor 4-Alpha; Hepatic Nuclear Factor 4 Alpha; HNF4alpha10/11/12
- Gene ID
- 3172
- UniProt ID
- P41235
- Cellular Localization
- Nucleus
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HNF4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2466z | Mouse Anti-HNF4A Recombinant Antibody (clone 35C6) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| MOB-2375MZ | Recombinant Mouse Anti-Human HNF4A Antibody | ICC, IF, WB | Mouse antibody |
| MRO-0757-CN | Rabbit Anti-HNF4A Recombinant Antibody (clone CBACN-280) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ57 | Mouse Anti-HNF4A Recombinant Antibody (VS3-CJ57) | WB, ELISA | Mouse IgG |
| VS3-CJ937 | Rabbit Anti-HNF4A Recombinant Antibody (VS3-CJ937) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1642 | Rabbit Anti-HNF4A Recombinant Antibody (clone DS1642AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3225 | Anti-HNF4A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3226 | Anti-Mouse HNF4A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3227 | Anti-Rat HNF4A Immunohistochemistry Kit | IHC |
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