Recombinant Rabbit Anti-IL10 Capture Antibody (clone R03-1G3) (CAT#: VS3-WK1708)
This antibody is a rabbit recombinant antibody against human IL10. It was developed by immunization of rabbit with recombinant human IL10 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R03-1G3 and R09-8O6. The clone R03-1G3 can be used as a capture antibody in ELISA analysis to identify human IL10.
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_B_9_5.jpg
Figure 2 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_A_6_6.jpg
Figure 3 RNA cell line category: Group enriched (HMC-1, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
Figure 3 RNA cell line category: Group enriched (HMC-1, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
Specifications
- Immunogen
- Recombinant human IL10 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL10
- Species Reactivity
- Human
- Clone
- R03-1G3
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Interleukin 10
- Gene ID
- 3586
- UniProt ID
- P22301
- Long Name
- Interleukin 10
- Sequence Similarities
- Belongs to the IL-10 family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_000563.1
- Function
- Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-080 | Rat Anti-IL10 Recombinant Antibody (clone 9D7); Fab Fragment | Neut | Rat Fab |
| HPAB-0223CQ-F(E) | Mouse Anti-IL10 Recombinant Antibody (clone B-N10); Fab Fragment | ELISA, FC, Block | Mouse Fab |
| HPAB-0224CQ-F(E) | Human Anti-IL10 Recombinant Antibody (clone BT-063); Fab Fragment | ELISA, FC, Block | Humanized Fab |
| HPAB-0842-FY-S(P) | Human Anti-IL10 Recombinant Antibody; scFv Fragment (HPAB-0842-FY-S(P)) | ELISA | Humanized scFv |
| HPAB-0843-FY-S(P) | Human Anti-IL10 Recombinant Antibody; scFv Fragment (HPAB-0843-FY-S(P)) | ELISA | Humanized scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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