Recombinant Rabbit Anti-IL10 Detection Antibody (clone R07-3Q4) (CAT#: VS3-WK1787)
This antibody is a rabbit recombinant antibody against rat IL10. It was developed by immunization of rabbit with recombinant rat IL10 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R07-7Q9 and R07-3Q4 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R07-3Q4 can be used in ELISA analysis to identify rat IL10.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_B_9_5.jpg
Figure 2 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_A_6_6.jpg
Figure 3 RNA cell line category: Group enriched (HMC-1, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
Figure 3 RNA cell line category: Group enriched (HMC-1, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
Specifications
- Immunogen
- Recombinant rat IL10 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Rat IL10
- Species Reactivity
- Rat
- Clone
- R07-3Q4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- If2a; IL10X
- Gene ID
- 25325
- UniProt ID
- P29456
- Long Name
- Interleukin 10
- Sequence Similarities
- Belongs to the IL-10 family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_036986.2
- Function
- Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity).
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Related Resources
Asthma
Inflammatory Bowel Diseases
Systemic Lupus Erythematosus
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL10"
Select a product category from the dropdown menu below to view related products.
Please select product type
Fab Antibody Products
ScFv Antibody Products
Chicken IgY Antibody Products
Blocking Antibody Products
IgM Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
Epitope-Specific Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-080 | Rat Anti-IL10 Recombinant Antibody (clone 9D7); Fab Fragment | Neut | Rat Fab |
| HPAB-0022-YC-F(E) | Human Anti-IL10 Recombinant Antibody; Fab Fragment (HPAB-0022-YC-F(E)) | ELISA, Neut | Humanized Fab |
| HPAB-0223CQ-F(E) | Mouse Anti-IL10 Recombinant Antibody (clone B-N10); Fab Fragment | ELISA, FC, Block | Mouse Fab |
| HPAB-0224CQ-F(E) | Human Anti-IL10 Recombinant Antibody (clone BT-063); Fab Fragment | ELISA, FC, Block | Humanized Fab |
| HPAB-0344-WJ-F(E) | Human Anti-IL10 Recombinant Antibody (clone 12G8); Fab Fragment | ELISA | Humanized Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-WK1787. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-1.png)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
-1.png)
Application: ELISA, FC, Block, SPR
Application: ELISA
Application: ELISA
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, Neut
Application: ELISA, Inhib, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
