Recombinant Rabbit Anti-ITGA5 Antibody (clone R02-3G4) (CAT#: VS3-FY770)
This product is a recombinant rabbit antibody that recognizes ITGA5. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R02-3G4 is specific for human, mouse ITGA5. The antigen is synthetic peptide of human integrin α5.
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WB
Figure 1. Recombinant Rabbit Anti-ITGA5 Antibody (clone R02-3G4) in WB.
Western Blot analysis of Integrin alpha 5 in K562, 3T3 lysates using Integrin alpha 5 antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-ITGA5 Antibody (clone R02-3G4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin alpha 5 Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human integrin α5.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse ITGA5
- Species Reactivity
- Human, Mouse
- Clone
- R02-3G4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 115 kDa; Observed MW: 150 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Integrin Subunit Alpha 5
- Gene ID
- 3678
- UniProt ID
- P08648
- Sequence Similarities
- Belongs to the integrin alpha chain family.
- Cellular Localization
- Cell junction, Membrane
- Post Translation Modifications
- Proteolytic cleavage by PCSK5 mediates activation of the precursor.
- Protein Refseq
- NP_002196.4
- Function
- Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).
ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).
(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.
(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.
(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Recommended Products
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1727z | Mouse Anti-ITGA5 Recombinant Antibody (clone 11B1) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-0506CT | Recombinant Mouse anti-Human ITGA5 Monoclonal antibody (DC50f) | FC | |
| MRO-0849-CN | Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (CBACN-310) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-QX607 | Mouse Anti-ITGA5 Recombinant Antibody (clone 10F6) | WB, IHC, ICC, FC, ELISA | Mouse IgG2a |
| VS3-CJ513 | Rabbit Anti-ITGA5 Recombinant Antibody (VS3-CJ513) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
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