Recombinant Rabbit Anti-MAPK10 Antibody (clone 29E10)
CAT#: VS4-CJ27
This rabbit anti-MAPK10 antibody (clone 29E10) is a product that recognizes human, mouse MAPK10. The immunogen of this antibody is a synthesized peptide derived from human MAPK10. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22625/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22625/48633_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22625/48633_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22625/48633_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (AF22)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109339-MAPK10
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Specifications
- Immunogen
- A synthesized peptide derived from human MAPK10
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse MAPK10
- Species Reactivity
- Human, Mouse
- Clone
- 29E10
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
- Gene ID
- 5602
- UniProt ID
- P53779
- Long Name
- Mitogen-activated protein kinase 10
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Nucleus
- Post Translation Modifications
- Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
Palmitoylation regulates subcellular location and axonal development.
- Protein Refseq
- NP_001304996.1; NP_001304997.1; NP_001304998.1
- Function
- Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692).
Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
| MOB-2130z | Mouse Anti-MAPK10 Recombinant Antibody (clone 11A7) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1199CT | Recombinant Mouse anti-Human MAPK10 Monoclonal antibody (3H23I4) | ELISA, WB | |
| VS7-0425-WR348 | Mouse Anti-MAPK10 Recombinant Antibody (clone 2B10H12) | WB | Mouse IgG2a |
| VS7-0425-WR349 | Mouse Anti-MAPK10 Monoclonal Antibody (clone 10E4A4) | WB, ICC | Mouse IgG1 |
| VS7-0425-WR350 | Mouse Anti-MAPK10 Monoclonal Antibody (VS7-0425-WR350) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2149 | Hi-Affi™ Recombinant Rabbit Anti-MAPK10 Monoclonal Antibody (DS2149AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1297 | Anti-MAPK10 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4227 | Anti-Human MAPK10 Immunohistochemistry Kit | IHC |
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