Recombinant Rabbit Anti-MCAM Antibody (clone R05-5A2) (CAT#: VS3-FY905)
This product is a recombinant rabbit antibody that recognizes MCAM. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-5A2 is specific for human, rat MCAM. The antigen is synthetic peptide of human cd146.
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Custom Production
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WB
Figure 1. Recombinant Rabbit Anti-MCAM Antibody (clone R05-5A2) in WB.
Western Blot analysis of CD146 in C6, Hela lysates using CD146 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-MCAM Antibody (clone R05-5A2) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD146 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human CD146.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat MCAM
- Species Reactivity
- Human, Rat
- Clone
- R05-5A2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 72 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Melanoma Cell Adhesion Molecule; S-Endo 1 Endothelial-Associated Antigen; Melanoma-Associated Antigen MUC18; Cell Surface Glycoprotein P1H12; Melanoma-Associated Antigen A32; Gicerin
- Gene ID
- 4162
- UniProt ID
- P43121
- Cellular Localization
- Membrane
- Protein Refseq
- NP_006491.2
- Function
- Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-516CL | Anti-Human MCAM Recombinant Antibody (ABX-MA1) | WB, FuncS | Antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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