Recombinant Rabbit Anti-MMP14 Antibody (clone R05-6H5)
CAT#: VS3-FY938
This product is a recombinant rabbit antibody that recognizes MMP14. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R05-6H5 is specific for human, Hamster MMP14. The antigen is synthetic peptide of human mmp14.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/1591_G3_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong luminal membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/134761_A_8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows positivity in stromal cells and plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/1658_G1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, HHSteC, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000157227-MMP14
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WB
Figure 1. Recombinant Rabbit Anti-MMP14 Antibody (clone R05-6H5) in WB.
Western Blot analysis of MMP14 in CHO-K1 lysates using MMP14 antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-MMP14 Antibody (clone R05-6H5) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using an antibody to MMP14. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human MMP14.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster MMP14
- Species Reactivity
- Human, Hamster
- Clone
- R05-6H5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 66 kDa; Observed MW: 66 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Matrix Metallopeptidase 14; Matrix Metallopeptidase 14 (Membrane-Inserted); Membrane-Type-1 Matrix Metalloproteinase; Membrane Type 1 Metalloprotease; EC 3.4.24.80; MT-MMP 1; MT1-MMP; MMP-14; MMP-X1
- Gene ID
- 4323
- UniProt ID
- P50281
- Sequence Similarities
- Belongs to the peptidase M10A family.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- The precursor is cleaved by a furin endopeptidase.
Tyrosine phosphorylated by PKDCC/VLK.
- Protein Refseq
- NP_004986.1
- Function
- Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity).
May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484).
Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657).
Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).
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Related Signaling Pathways
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-0822CL-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (TAB-0822CL-F(E)) | ELISA, FC, IHC | Human Fab |
| TAB-0823CL-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (TAB-0823CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-279 | Mouse Anti-MMP14 Recombinant Antibody (clone LEM-2/15); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| HPAB-0260CQ-F(E) | Human Anti-MMP14 Recombinant Antibody; Fab Fragment (HPAB-0260CQ-F(E)) | ELISA, FC, IHC | Human Fab |
| HPAB-0887-FY-S(P) | Mouse Anti-MMP14 Recombinant Antibody (clone 66C); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0888-FY-S(P) | Mouse Anti-MMP14 Recombinant Antibody (clone 19B); Fab Fragment | ELISA, IA | Mouse Fab |
| HPAB-0889-FY-S(P) | Mouse Anti-MMP14 Recombinant Antibody (clone MMP98); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1237CT | Recombinant Mouse anti-Human MMP14 Monoclonal antibody (EML768) | IHC-P, WB | |
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| HPAB-0888-FY | Mouse Anti-MMP14 Recombinant Antibody (clone 19B) | ELISA, IA | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2251 | Hi-Affi™ Recombinant Rabbit Anti-MMP14 Monoclonal Antibody (DS2251AB) | IHC-P, ICC, IF, FC, IP, WB | IgG |
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|---|---|---|---|
| VS3-CJ1005 | Mouse Anti-MMP14 (C-Terminus) Recombinant Antibody (VS3-CJ1005) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC295 | Recombinant Anti-MMP14 Vesicular Antibody, EV Displayed (VS-0425-YC295) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4447 | Anti-MMP14 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4448 | Anti-Mouse MMP14 Immunohistochemistry Kit | IHC |
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