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Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5)

Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5) (CAT#: VS3-FY1091)

Name: Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5)
SKU: VS3-FY1091
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes PAK1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R08-5I5 is specific for human, mouse, rat PAK1. The antigen is synthetic peptide of human pak1.

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1. Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5) in ICC.

Figure 1. Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5) in ICC.

Immunocytochemical analysis of PAK1 (green) in Jurkat using PAK1 antibody and DAPI (blue).

Figure 2. Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5) in WB.

Figure 2. Recombinant Rabbit Anti-PAK1 Antibody (clone R08-5I5) in WB.

Western Blot analysis of PAK1 in C6 lysates using PAK1 antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/if_selected.jpg

Normal Tissue

Figure 2 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/28952_A_6_6.jpg

Figure 3 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/28952_A_9_8.jpg

Figure 4 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/28952_B_8_5.jpg

Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/28952_A_8_3.jpg

Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/28952_A_7_4.jpg

Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3565/28952_A_7_5.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149269-PAK1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human PAK1.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat PAK1

Species Reactivity
Human, Mouse, Rat

Clone
R08-5I5

Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 61 kDa; Observed MW: 61 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
P21 (RAC1) Activated Kinase 1; P21/Cdc42/Rac1-Activated Kinase 1 (STE20 Homolog, Yeast); P21/Cdc42/Rac1-Activated Kinase 1 (Yeast Ste20-Related); P21 Protein (Cdc42/Rac)-Activated Kinase 1; STE20 Homolog, Yeast; EC 2.7.11.1; Alpha-PAK

Gene ID
5058

UniProt ID
Q13153

Cellular Localization
Cell junction, Cell membrane, Cell projection, Chromosome, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Secondary Antibody

Isotype Control

Related Resources

Related Diseases
Related Signaling Pathways

Bladder Cancer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "PAK1"

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Please select product type

IgG Antibody Products Rabbit Monoclonal Antibody Products Antibody Magnetic Beads

CAT	Product Name	Application	Type
MOB-2175z	Mouse Anti-PAK1 Recombinant Antibody (clone 3A7)	WB, ELISA, IHC	Mouse IgG1, κ
MOB-1535CT	Recombinant Mouse anti-Human PAK1 Monoclonal antibody (EML2308)	IHC-P, WB
ZG-0495U	Rabbit Anti-PAK1 Recombinant Antibody (clone 4F10)	ELISA, WB, IF	Rabbit IgG
VS3-CJ183	Rabbit Anti-PAK1 Recombinant Antibody (VS3-CJ183)	WB, ICC, IF, IHC, IP, FC	Rabbit IgG
VS3-FY1090	Recombinant Rabbit Anti-PAK1 Antibody (clone R02-5G3)	WB, IHC-Fr, IHC-P, ICC, IF, IP	Rabbit IgG

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