Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R01-4B5)
CAT#: VS3-FY1284
This product is a recombinant rabbit antibody that recognizes RPS6KB1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-4B5 is specific for human, mouse, rat RPS6KB1. The antigen is synthetic peptide of human s6k1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39442/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3838/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18346/42029_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108443-RPS6KB1
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WB
Figure 1. Recombinant Rabbit Anti-RPS6KB1 Antibody (clone R01-4B5) in WB.
Western Blot analysis of S6K1 in rat brain lysates using an S6K1 antibody.
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Specifications
- Immunogen
- Synthetic peptide of human S6K1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RPS6KB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-4B5
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 59 kDa; Observed MW: 70 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ribosomal Protein S6 Kinase B1; Serine/Threonine-Protein Kinase 14A; Ribosomal Protein S6 Kinase I; EC 2.7.11.1; S6K-Beta-1; P70 S6KA; STK14A; S6K1; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1; 70 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Beta-1; Serine/Threonine Kinase 14 Alpha
- Gene ID
- 6198
- UniProt ID
- P23443
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RPS6KB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3935z | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 30G9) | WB, ELISA, FC, IHC | Mouse IgG1 |
| VS3-QX985 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 3A2-2E7-2D4) | IHC | Mouse IgG1 |
| VS3-QX986 | Mouse Anti-RPS6KB1 Recombinant Antibody (clone 8H8) | IHC | Mouse IgG1 |
| VS3-XY3490 | Rabbit Anti-RPS6KB1 Recombinant Antibody (clone R07-7V3) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS3-WK2109 | Rabbit Anti-RPS6KB1 (pT421/pS424) Recombinant Antibody (clone R09-3B7) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3126 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (DS3126AB) | IHC-P | IgG |
| MOR-4660 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH174DS) | WB, IF, ICC, FC | IgG |
| MOR-4699 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH213DS) | WB, IF, ICC, FC | IgG |
| MOR-4700 | Hi-Affi™ Recombinant Rabbit Anti-RPS6KB1 Monoclonal Antibody (TH214DS) | WB, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1968 | Anti-RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6231 | Anti-Mouse RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6230 | Anti-Human RPS6KB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6232 | Anti-Rat RPS6KB1 Immunohistochemistry Kit | IHC |
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