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    Recombinant Rabbit Anti-S100A4 Capture Antibody (clone R54-1P-4) (CAT#: VS3-WK1922)

    This antibody is a rabbit recombinant antibody against human S100A4. It was developed by immunization of rabbit with recombinant human S100A4 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R54-1P-4 and R62-2M-9. The clone R54-1P-4 can be used as a capture antibody in ELISA analysis to identify human S100A4.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A549
    Figure 1 IF staining of human cell line A549
    (Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
    Normal Tissue
    Figure 2 Lung.
    Figure 2 Lung.
    (High expression in lung)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
    Figure 3 Spleen
    Figure 3 Spleen
    (High expression in spleen)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
    Figure 5 Testis
    Figure 5 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
    Figure 6 Placenta
    Figure 6 Placenta
    (Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
    Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4

    Specifications

    • Immunogen
    • Recombinant human S100A4 protein
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human S100A4
    • Species Reactivity
    • Human
    • Clone
    • R54-1P-4
    • Applications
    • ELISA
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • 0.01M PBS, pH 7.2
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Target

    • Alternative Names
    • S100 Calcium Binding Protein A4; S100 Calcium-Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Placental Calcium-Binding Protein; Fibroblast-Specific Protein-1; Protein Mts1; MTS1; CAPL; S100 Calcium Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Leukemia Multidrug Resistance Associated Protein; Malignant Transformation Suppression 1
    • Long Name
    • S100 Calcium Binding Protein A4
    • Sequence Similarities
    • Belongs to the S-100 family.
    • Cellular Localization
    • Cytoplasm, Nucleus, Secreted
    • Function
    • Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy.
      Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9.

    Recommended Products

    Detection antibody

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    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    Humanized Antibody Products Mouse Antibody Products Single Domain Antibody Products IgG Antibody Products Rabbit Monoclonal Antibody Products Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    TAB-546CL Human Anti-S100A4 Recombinant Antibody (TAB-546CL) ELISA Human IgG

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