Recombinant Rabbit Anti-S100A4 Detection Antibody (clone R62-2M-9) (CAT#: VS3-WK1923)
This antibody is a rabbit recombinant antibody against human S100A4. It was developed by immunization of rabbit with recombinant human S100A4 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R54-1P-4 and R62-2M-9 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R62-2M-9 can be used in ELISA analysis to identify human S100A4.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
Figure 2 Lung.
(High expression in lung)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
(High expression in lung)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
Figure 3 Spleen
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
Figure 6 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
Specifications
- Immunogen
- Recombinant human S100A4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A4
- Species Reactivity
- Human
- Clone
- R62-2M-9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein A4; S100 Calcium-Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Placental Calcium-Binding Protein; Fibroblast-Specific Protein-1; Protein Mts1; MTS1; CAPL; S100 Calcium Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Leukemia Multidrug Resistance Associated Protein; Malignant Transformation Suppression 1
- Gene ID
- 6275
- UniProt ID
- P26447
- Long Name
- S100 Calcium Binding Protein A4
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Protein Refseq
- NP_002952.1; NP_062427.1
- Function
- Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy.
Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100A4"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3568z | Mouse Anti-S100A4 Recombinant Antibody (clone 24D3) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| TAB-378CT | Anti-Human S100A4 Recombinant Antibody (TAB-378CT) | FuncS, IHC, Neut, Inhib | Mouse IgG1, κ |
| TAB-378CT-S(P) | Anti-Human S100A4 Recombinant Antibody scFv Fragment (5C3) | WB, IHC, Block | |
| VS3-FY1293 | Recombinant Mouse Anti-S100A4 Antibody (clone 3A1B2) | ELISA, IHC, FC | Mouse IgG1 |
| VS-0224-XY90 | Rat Anti-Mouse S100a4 Recombinant Antibody (VS-0224-XY90) | ELISA, WB | Rat IgG2b |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-546CL | Human Anti-S100A4 Recombinant Antibody (TAB-546CL) | ELISA | Human IgG |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-377CT | Anti-Human S100A4 Therapeutic Single Domain Antibody (TAB-377CT) | WB, ELISA | Single domain antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-379CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-379CT) | WB, IHC, Block | Mouse IgG2a |
| TAB-380CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-380CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-381CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-381CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-382CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-382CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-379CT-F(E) | Mouse Anti-S100A4 Recombinant Antibody; Fab Fragment (TAB-379CT-F(E)) | WB, IHC, Block | Mouse Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3155 | Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) | WB, IHC, ICC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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