Recombinant Mouse Anti-TPO Antibody (clone 9G3-6F3-9B7)
CAT#: VS3-FY1477
This product is a recombinant mouse antibody that recognizes TPO. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 9G3-6F3-9B7 is specific for human TPO. The antigen is synthetic peptide derived from human thyroid peroxidase (tpo).
Gene Expression
Tested Data
Normal Tissue
Figure 1 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7987/21449_B_2_5.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115705-TPO/subcellular
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IHC-P
Figure 1. Recombinant Mouse Anti-TPO Antibody (clone 9G3-6F3-9B7) in IHC-P.
Immunohistochemical analysis of paraffin-embedded follicular thyroid carcinoma using thyroid peroxidase antibody. Antigen retrieval was performed using high pressure and high temperature sodium citrate pH 6.0.
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Specifications
- Immunogen
- Synthetic peptide derived from human thyroid peroxidase (TPO).
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human TPO
- Species Reactivity
- Human
- Clone
- 9G3-6F3-9B7
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Thyroid Peroxidase; EC 1.11.1.8; Thyroid Microsomal Antigen; Thyroperoxidase; EC 1.11.1; TDH2A; MSA; TPX
- Gene ID
- 7173
- UniProt ID
- P07202
- Sequence Similarities
- Belongs to the peroxidase family. XPO subfamily.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylated.
Heme is covalently bound through a H2O2-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part.
- Protein Refseq
- NP_000538.3; NP_001193673.1; NP_001193674.1
- Function
- Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.
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Related Diseases
Autoimmune Thyroid Disease
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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- Product Name
Isotype Control
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
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