Recombinant Rabbit Anti-VEGFA Antibody (clone R04-6D7)

Name: Recombinant Rabbit Anti-VEGFA Antibody (clone R04-6D7)
SKU: VS3-FY1543
Rating: 5 (1 reviews)

CAT#: VS3-FY1543

This product is a recombinant rabbit antibody that recognizes VEGFA. This antibody has been reported for use in Western Blot. The clone R04-6D7 is specific for human, mouse, rat VEGFA. The antigen is human vegfa synthetic peptide.

Gene Expression Tested Data

Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA

❮

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Figure 1. Recombinant Rabbit Anti-VEGFA Antibody (clone R04-6D7) in WB.

Western Blot analysis of VEGFA in RAW264.7 lysates using VEGFA antibody.

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Specifications

Immunogen
Human VEGFA Synthetic Peptide.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat VEGFA

Species Reactivity
Human, Mouse, Rat

Clone
R04-6D7

Applications
Western Blot

Conjugate
Unconjugated

MW
Calculated MW: 27 kDa; Observed MW: 25-50 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000

Target

Alternative Names
Vascular Endothelial Growth Factor A; Vascular Permeability Factor; VEGF; VPF; Vascular Endothelial Growth Factor A121; Vascular Endothelial Growth Factor A165; Vascular Endothelial Growth Factor; VEGF-A; MVCD1

Gene ID
7422

UniProt ID
P15692

Sequence Similarities
Belongs to the PDGF/VEGF growth factor family.

Cellular Localization
Secreted

Protein Refseq
NP_001020537.2; NP_001020538.2; NP_001020539.2

Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437).

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-FY1543.pdf

MSDS

MSDS - VS3-FY1543.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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