What is the structural basis of this scaffold protein?

The scaffold is the tenth fibronectin type III domain (10Fn3), a small autonomous folding unit with a beta-sandwich structure that closely resembles immunoglobulin domains. It occurs in many animal proteins involved in ligand binding.

What affinity does this variant have for PCSK9?

After affinity maturation, 10Fn3 variants were identified having affinities (KD=1.2 nM) for Proprotein convertase subtilisin kexin-9 (PCSK9). This value was determined by biosensor technology, validating its potential for diagnostic and research applications.

10-FnIII scaffold protein anti-Human Proprotein convertase subtilisin kexin-9 (PCSK9)

CAT#: SFN-L018

The tenth fibronectin type III domain (10Fn3) that bound Proprotein convertase subtilisin kexin-9 (PCSK9) was isolated from the library using phage display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=1.2 nM) for Proprotein convertase subtilisin kexin-9 (PCSK9) as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

RNA Expression Figure 1 RNA cell line category: Cell line enhanced (A-431, CACO-2, HaCaT, HeLa, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169174-PCSK9

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Specifications

Scaffold Name
10-FnIII

Origin
Fibronectin

Core Structure
β-sandwich of 7 β-strands

Variable Regions
2–3 Loops

Target
Proprotein convertase subtilisin kexin-9 (PCSK9)

Species Reactivity
Human

Expression Host
E. coli

Affinity Constant
1.2 nM

Applications
ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study

Target

Alternative Names
Fibronectin; 10-FnIII; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3

Gene ID
255738

UniProt ID
Q8NBP7

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High Affinity Binding

The affinity maturation of this 10Fn3 variant is impressive, with a KD of 1.2 nM. It binds Proprotein convertase subtilisin kexin-9 tightly, making it an excellent candidate for our biosensor-based interaction studies.

Robust Beta Sandwich Structure

We appreciate the structural stability of the fibronectin type III domain. Its beta-sandwich structure resembles immunoglobulin domains, providing a familiar yet distinct folding unit that performs reliably in our ligand binding assays.

Q&As

What is the structural basis of this scaffold protein?

A: The scaffold is the tenth fibronectin type III domain (10Fn3), a small autonomous folding unit with a beta-sandwich structure that closely resembles immunoglobulin domains. It occurs in many animal proteins involved in ligand binding.
What affinity does this variant have for PCSK9?

A: After affinity maturation, 10Fn3 variants were identified having affinities (KD=1.2 nM) for Proprotein convertase subtilisin kexin-9 (PCSK9). This value was determined by biosensor technology, validating its potential for diagnostic and research applications.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - SFN-L018.pdf

MSDS

MSDS - SFN-L018.pdf

COA

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