What is the host and expression system for TAB-H31?

This is a fully recombinant human antibody, meaning its origin is human. It is specified as a Human IgG type. It is produced in a mammalian CHO (Chinese Hamster Ovary) expression system to ensure it has the appropriate structure and post-translational modifications for research use.

What is the research significance of this antibody's target, PCSK9?

This antibody targets PCSK9 (Proprotein Convertase Subtilisin/Kexin type 9). PCSK9 is a critical enzyme in cholesterol homeostasis research. It functions by binding to the LDL receptor on the surface of liver cells, promoting its degradation. This action reduces the liver's ability to clear LDL-cholesterol from the bloodstream, making it a key target of study.

Human Anti-PCSK9 Recombinant Antibody (TAB-H31)

CAT#: TAB-H31

This product is a recombinant human antibody that recognizes PCSK9. The antibody was purified by affinity chromatography.

Tested Data Gene Expression

ELISA

Figure 1 Anti-PCSK9 Antibody (TAB-H31) in ELISA

ELISA analysis of TAB-H31 was performed by coating with Human PCSK9 Protein (His Tag). Then blocked with BSA and incubated with TAB-H31. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-PCSK9 Antibody (TAB-H31) in DB

Dot Blot analysis of TAB-H31 was performed by coating with Human PCSK9 Protein (His Tag).

TAB-H31 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 3000)

SDS-PAGE

Figure 3 Anti-Human PCSK9 Recombinant Antibody (TAB-H31) in SDS-PAGE

SDS-PAGE analysis of TAB-H31 in non-reduced (Lane 2) and reduced (Lane 1) conditions.

SEC-HPLC

Figure 4 Anti-Human PCSK9 Recombinant Antibody (TAB-H31) in HPLC

HPLC analysis of TAB-H31 shows the purity is>95%.

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RNA Expression Figure 1 RNA cell line category: Cell line enhanced (A-431, CACO-2, HaCaT, HeLa, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169174-PCSK9

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Specifications

Host Species
Human

Derivation
human

Type
Human IgG2, λ

Species Reactivity
Human

Applications
Suitable for use in FuncS, IF, Neut, ELISA, FC, IP, ICC and most other immunological methods.

MW
141.8 kDa

Related Disease
Hypercholesterolemia

Product Property

Purity
>95% as determined by SDS-PAGE

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Proprotein Convertase Subtilisin/Kexin Type 9; Subtilisin/Kexin-Like Protease PC9; NARC-1; NARC1; PC9; Convertase Subtilisin/Kexin Type 9 Preproprotein; Hypercholesterolemia, Autosomal Dominant 3; Neural Apoptosis Regulated Convertase 1; Neural Apoptosis-Regulated Convertase 1

Gene ID
353175

UniProt ID
Q8NBP7

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Breast cancer biomarkers at key points during disease progression

Human PCSK9 Antibody

For our research on lipid metabolism, we needed a fully human antibody against PCSK9. TAB-H31 fits this requirement perfectly. It's a recombinant human IgG expressed in CHO cells and is validated for a wide array of assays, including ELISA FuncS, IF, Neut, ELISA, FC, IP, ICC and most other immunological methods.

Broad Application Antibody

This anti-human PCSK9 antibody is incredibly versatile. We are using it for ELISA and Flow Cytometry, but the fact that it's also validated for IP and IF is a significant advantage. Having a single, reliable human IgG antibody for all these applications is a great find for our lab.

Q&As

What is the host and expression system for TAB-H31?

A: This is a fully recombinant human antibody, meaning its origin is human. It is specified as a Human IgG type. It is produced in a mammalian CHO (Chinese Hamster Ovary) expression system to ensure it has the appropriate structure and post-translational modifications for research use.
What is the research significance of this antibody's target, PCSK9?

A: This antibody targets PCSK9 (Proprotein Convertase Subtilisin/Kexin type 9). PCSK9 is a critical enzyme in cholesterol homeostasis research. It functions by binding to the LDL receptor on the surface of liver cells, promoting its degradation. This action reduces the liver's ability to clear LDL-cholesterol from the bloodstream, making it a key target of study.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-H31, RRID: AB_3112029)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-H31.pdf

MSDS

MSDS - TAB-H31.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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