What research applications are validated for the PCSK9 antibody?

Our PCSK9 antibody has been validated in Western blot, flow cytometry, immunoprecipitation, and ELISA applications. It is designed to reliably detect PCSK9 with high specificity, making it a versatile reagent in studies focused on protein interactions and molecular signaling pathways.

How do quality control measures contribute to its experimental reliability?

Produced in CHO cells with rigorously controlled processes, the antibody exhibits over 95% purity as measured by RP-HPLC and SDS-PAGE. This high level of quality ensures minimal background and reproducibility across multiple assays, making it an excellent choice for PCSK9 research.

Anti-Human PCSK9 Recombinant Antibody (Bococizumab)

CAT#: TAB-H10

Recombinant Humanized (from mouse) antibody to Human PCSK9. Bococizumab (codenamed RN316)is a monoclonal antibody against PCSK9 designed to reduce LDL cholesterol

Tested Data Published Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human PCSK9 Recombinant Antibody (TAB-H10) in SDS-PAGE

Lane 1: Non-reduced
Lane 2: Reduced

SEC-HPLC

Figure 2 Anti-Human PCSK9 Recombinant Antibody (TAB-H10) in HPLC

The purity of TAB-H10 was greater than 95% as determined by SEC-HPLC.

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Neut

Figure 1 Effect of Antidrug Antibodies on the Durability of Low-Density Lipoprotein (LDL) Cholesterol Lowering and on Plasma Bococizumab and PCSK9 Levels.

Panel A shows the effect of generalized antidrug antibodies (ADAs) on LDL cholesterol levels among the patients who received placebo and among those who received bococizumab, according to whether the patients were ADA-negative throughout the trial, had ADA titers in the lowest two thirds of maximum titers (<1:1176) during follow-up, had ADA titers in the highest third of titers (≥1:1176) during follow-up, or were in the subgroup with ADA titers in the top 10% (≥1:5674). The light blue bars indicate prevalent rates of antibodies at each time point, and the dark blue bars the cumulative incidence of antibody positivity during follow-up. Panel B shows plasma bococizumab levels and Panel C total plasma proprotein convertase subtilisin–kexin type 9 (PCSK9) levels over time, according to the ADA titer. Panel D shows the effect of the neutralizing antibody (NAb) titer on LDL cholesterol levels.

Ridker, P. M., Tardif, J. C., Amarenco, P., Duggan, W., Glynn, R. J., Jukema, J. W.,... & Revkin, J. (2017). Lipid-reduction variability and antidrug-antibody formation with bococizumab. New England Journal of Medicine, 376(16), 1517-1526.

Neut

Figure 2 Variability in the Reduction in LDL Cholesterol Levels, According to the Presence or Absence of Antidrug Antibodies (ADAs).

Waterfall plots for individual patients who received bococizumab show the percent change from baseline in LDL cholesterol levels at 12 weeks (Panel A) and at 52 weeks among the patients in whom antidrug antibodies developed (Panel B) and among those in whom antidrug antibodies did not develop (Panel C). Data are limited to the patients who reported having received bococizumab in the 21 days before the measurement of LDL cholesterol.

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RNA Expression Figure 1 RNA cell line category: Cell line enhanced (A-431, CACO-2, HaCaT, HeLa, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169174-PCSK9

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Specifications

Host Species
Human

Derivation
Humanized (from mouse)

Type
IgG2 - kappa

Specificity
PCSK9(proprotein convertase subtilisin/kexin type 9, neural apoptosis-regulated convertase 1, NARC1, NARC-1, proproteine convertase 9, PC9) [Homo sapiens]

Species Reactivity
Human

Applications
WB, FC, IP, ELISA, Neut, FuncS, IF

CAS
1407495-02-6

Generic Name
bococizumab

ChEMBL
CHEMBL3137349

MW
145.1 kDa

Related Disease
Hypercholesterolemia

Product Property

Purity
>95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.

Applications

Application Notes
The PCSK9 antibody has been reported in applications of WB, FC, IP, ELISA, Neut, FuncS, IF.

Target

Alternative Names
bococizumab;1407495-02-6;PF-04950615;RN316;RN-316;L1L3;PCSK9;proprotein convertase subtilisin/kexin type 9;HCHOLA3, hypercholesterolemia, autosomal dominant 3;FH3;NARC 1;NARC1;NARC-1;HCHOLA3;

Gene ID
353175

UniProt ID
Q8NBP7

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Breast cancer biomarkers at key points during disease progression

Excellent PCSK9 Tool

I have been using the PCSK9 antibody in Western blot and ELISA assays. Its humanized design, combined with over 95% purity confirmed by RP-HPLC and SDS-PAGE, has significantly improved the reproducibility of my experiments. The detailed protocol included with the product made it very user-friendly.

High Purity Reagent

The anti-PCSK9 antibody has delivered consistent performance in flow cytometry and immunoprecipitation. Detailed information on production and storage boosted my confidence in using it for research purposes. The high purity and recombinant humanized design ensure that experimental outcomes are reliable and reproducible.

Q&As

What research applications are validated for the PCSK9 antibody?

A: Our PCSK9 antibody has been validated in Western blot, flow cytometry, immunoprecipitation, and ELISA applications. It is designed to reliably detect PCSK9 with high specificity, making it a versatile reagent in studies focused on protein interactions and molecular signaling pathways.
How do quality control measures contribute to its experimental reliability?

A: Produced in CHO cells with rigorously controlled processes, the antibody exhibits over 95% purity as measured by RP-HPLC and SDS-PAGE. This high level of quality ensures minimal background and reproducibility across multiple assays, making it an excellent choice for PCSK9 research.

View the frequently asked questions answered by Creative Biolabs Support.

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-H10, RRID: AB_3112024)

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Biosimilar Overview

Please refer to Bococizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Bococizumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-H10.pdf

MSDS

MSDS - TAB-H10.pdf

COA

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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "bococizumab"

DrugMonitor™ Anti-Bococizumab Antibody (VS-1224-YC212)
Bococizumab has been used in trials for Dyslipidemia, Hyperlipidemia, Hypercholesterolemia, Cardiovascular Disease, and Heterozygous Familial Hypercholesterolemia treatment and prevention. The DrugMonitor™ Anti-Bococizumab Antibody (VS-1224-YC212) is an anti-drug antibody (ADA) against Bococizumab. This drug-based antibody is raised in mice immunized with the Bococizumab. The anti-Bococizumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Bococizumab in samples.

Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H10)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant Humanized (from mouse) antibody to Human PCSK9. It is a monoclonal antibody against PCSK9 designed to reduce LDL cholesterol

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PFBL-294	Human Anti-PCSK9 Recombinant Antibody (clone mAb1); Fab Fragment	WB, Block, FuncS	Human Fab

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PABZ-101	Human Anti-PCSK9 Recombinant Antibody (clone 1D05)	ELISA, WB, Neut	Human IgG2

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PFBZ-101	Human Anti-PCSK9 Recombinant Antibody (clone 1D05); Fab Fragment	ELISA, WB, Neut	Human Fab

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PFBL-671	Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16); Fab Fragment	WB, ELISA, FuncS	Mouse Fab

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PFBL-672	Human Anti-PCSK9 Recombinant Antibody (clone CBL36Q); Fab Fragment	ELISA, WB	Human Fab

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PSBZ-101	Human Anti-PCSK9 Recombinant Antibody (clone 1D05); scFv Fragment	ELISA, WB, Neut	Human scFv

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PSBL-670	Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (PSBL-670)	WB, FuncS	Human scFv

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PSBL-671	Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16); scFv Fragment	WB, ELISA, FuncS	Mouse scFv

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PSBL-672	Human Anti-PCSK9 Recombinant Antibody (clone CBL51Q); scFv Fragment	ELISA, WB	Human scFv

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TAB-0953CL	Human Anti-PCSK9 Recombinant Antibody (TAB-0953CL)	ELISA	Human IgG1

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TAB-0954CL	Human Anti-PCSK9 Recombinant Antibody (TAB-0954CL)	WB	Human IgG

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TAB-0955CL	Mouse Anti-PCSK9 Recombinant Antibody (TAB-0955CL)	WB	Mouse IgG

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TAB-0956CL	Anti-Human PCSK9 Recombinant Antibody (m7F8)	ELISA

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TAB-0957CL	Anti-Human PCSK9 Recombinant Antibody (h7F8)	ELISA	Humanized antibody

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TAB-0959CL	Human Anti-PCSK9 Recombinant Antibody (TAB-0959CL)	ELISA, FC	Human antibody

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TAB-0960CL	Anti-Human PCSK9 Recombinant Antibody (Ab1)	ELISA	Rabbit mAb

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TAB-0956CL-S(P)	Anti-Human PCSK9 Recombinant Antibody scFv Fragment (m7F8)	ELISA

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TAB-0960CL-S(P)	Anti-Human PCSK9 Recombinant Antibody scFv Fragment (Ab1)	ELISA	Rabbit mAb

CAT	Product Name	Application	Type
TAB-0956CL-F(E)	Anti-Human PCSK9 Recombinant Antibody Fab Fragment (m7F8)	ELISA

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TAB-0080CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0080CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

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TAB-0081CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0081CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
TAB-0082CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0082CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
TAB-0083CL-VHH	Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0083CL-VHH)	FuncS, Inhib, FC, WB	Single domain antibody

CAT	Product Name	Application	Type
NEUT-1826CQ	Recombinant Human Anti-PCSK9 Antibody, Unconjugated	Neut	IgG1

CAT	Product Name	Application	Type
MOR-2618	Hi-Affi™ Recombinant Rabbit Anti-PCSK9 Monoclonal Antibody (DS2618AB)	WB	IgG

CAT	Product Name	Application	Type
AFC-TAB-758	Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-758)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-H10	Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H10)	FC, IP, ELISA, Neut, FuncS	ADCC enhanced antibody

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AFC-TAB-H59	Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H59)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody

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AFC-TAB-H31	Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced	FC, IP, ELISA, Neut, FuncS, IF	Human IgG1, λ

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VS-0424-XY208	AbPlus™ Anti-PCSK9 Magnetic Beads (VS-0424-XY208)	IP, Protein Purification

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VS-0325-FY208	Human Anti-PCSK9 scFv-Fc Chimera (VS-0325-FY208)	WB, Block	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY5250	Anti-PCSK9 Immunohistochemistry Kit	IHC

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VS-0825-YC18	SmartAb™ Recombinant Anti-PCSK9 pH-dependent Antibody (Clone Bococizumab)	WB, FC, IP, ELISA, Neut, IF	Human IgG2 kappa

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VS-0825-YC277	SmartAb™ Recombinant Anti-PCSK9 pH-dependent Antibody (Clone Lodelcizumab)	ELISA, FC, IP IF, Neut, ICC	Human IgG1 kappa

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