Anti-Human ADAM17 Recombinant Antibody (D1(A12)) (CAT#: TAB-0669CLV)

The anti-ADAM17 antibody D1(A12) targets ADAM17. ADAM17 is also known as TACE and is responsible for cleaving a variety of pathologically significant substrate. D1(A12) can be used in the treatment of cancers such as ovarian cancer and colorectal cancer.


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ELISA

Figure 1 In vitroactivity of D1(A12) antibody.

Figure 1 In vitroactivity of D1(A12) antibody.

D1(A12) IgG inhibits PMA-induced shedding of ADAM17 substrates into IGROV1-Luc cellculture medium. Medium was collected 90 minutes after addition of PMA (100 ng/ml), D1(A12) IgG (200 nM) or solvent control. The proteins werequantified by ELISA.

Richards, F. M., Tape, C. J., Jodrell, D. I., & Murphy, G. (2012). Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PloS one, 7(7), e40597.

PK

Figure 2 The IGROV1-Luc xenograft model and D1(A12) pharmacokinetics.

Figure 2 The IGROV1-Luc xenograft model and D1(A12) pharmacokinetics.

Plasma and ascites fluid concentrations in IGROV1-Luc tumour-bearing mice. (D) D1(A12) IgG in mouse plasma: capacity forbinding to ADAM17 and FccR1. Plasma was collected at the indicated times after dosing the mice with a single dose of 10 mg/kg D1(A12). Bindingactivity in vitro was compared to the binding capacity of D1A12 stock solution.

Richards, F. M., Tape, C. J., Jodrell, D. I., & Murphy, G. (2012). Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PloS one, 7(7), e40597.

Inhib

Figure 3 Inhibition of IGROV1-Luc tumour growthin vivobyD1(A12) IgG.

Figure 3 Inhibition of IGROV1-Luc tumour growthin vivobyD1(A12) IgG.

Growth of IGROV1-Luc i.p. xenografts in mice dosedweekly with vehicle, 10 mg/kg infliximab or 10 mg/kg D1(A12),measured by bioluminescence. The mean and standard deviation ofday 32 tumour burden is shown, expressed as Avg Radiance.

Richards, F. M., Tape, C. J., Jodrell, D. I., & Murphy, G. (2012). Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PloS one, 7(7), e40597.

IHC

Figure 4 Distribution of D1(A12) and infliximab IgG in vivo at the end of the efficacy study.

Figure 4 Distribution of D1(A12) and infliximab IgG in vivo at the end of the efficacy study.

Immunohistochemistry to detect human IgG in IGROV1-Luc tumour and liver.

Richards, F. M., Tape, C. J., Jodrell, D. I., & Murphy, G. (2012). Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PloS one, 7(7), e40597.


Specifications

  • Immunogen
  • Recombinant human TACE ectodomain
  • Host Species
  • Human
  • Type
  • Human antibody
  • Specificity
  • Human
  • Clone
  • D1(A12)
  • Applications
  • ELISA, PK, Inhib, IHC
  • Related Disease
  • Ovarian cancer, colorectal cancer

Applications

  • Application Notes
  • The ADAM17 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, PK, Inhibition, Immunohistochemistry.

Target

  • Alternative Names
  • ADAM17; ADAM metallopeptidase domain 17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Fab Fragment Antibody

scFv Fragment Antibody

CAT Product Name Application Type
MOB-38-S(P) Recombinant Anti-human ADAM17 Antibody scFv Fragment ELISA, WB, IP, FuncS scFv
MHH-38-S(P) Recombinant Human Anti-human ADAM17 Antibody scFv Fragment IP, IF, Biosensors, FuncS scFv

Chimeric Antibody

CAT Product Name Application Type
TAB-0445CL Anti-Human ADAM17 Recombinant Antibody (cl022C3) ELISA, Inhib, FuncS Chimeric (mouse/human)
TAB-0445CL-S(P) Anti-Human ADAM17 Recombinant Antibody scFv Fragment (cl022C3) ELISA, Inhib, FuncS Chimeric (mouse/human)
TAB-0445CL-F(E) Anti-Human ADAM17 Recombinant Antibody Fab Fragment (cl022C3) ELISA, Inhib, FuncS Chimeric (mouse/human)

Humanized Antibody

CAT Product Name Application Type
TAB-0446CL Anti-Human ADAM17 Recombinant Antibody (hzl022C3) ELISA, Inhib, FuncS Humanized Antibody
TAB-0446CL-S(P) Anti-Human ADAM17 Recombinant Antibody scFv Fragment (hzl022C3) ELISA, Inhib, FuncS Humanized Antibody
TAB-0446CL-F(E) Anti-Human ADAM17 Recombinant Antibody Fab Fragment (hzl022C3) ELISA, Inhib, FuncS Humanized Antibody

Human Antibody

CAT Product Name Application Type
TAB-0447CL Anti-Human ADAM17 Recombinant Antibody (hl022C3) ELISA, Inhib, FuncS Human antibody
TAB-0447CL-S(P) Anti-Human ADAM17 Recombinant Antibody scFv Fragment (hl022C3) ELISA, Inhib, FuncS Human antibody
TAB-0447CL-F(E) Anti-Human ADAM17 Recombinant Antibody Fab Fragment (hl022C3) ELISA, Inhib, FuncS Human antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0070 Hi-Affi™ Rabbit Anti-ADAM17 Recombinant Antibody (clone DS70AB) WB, IF, IP, FC Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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