Human Anti-CD27 Recombinant Antibody (MOB-096LC) (CAT#: MOB-096LC)
This product is a recombinant human antibody that recognizes human CD27. The antibody was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 1 Anti-CD27 Recombinant Antibody (MOB-096LC) in ELISA
ELISA analysis of MOB-096LC was performed by coating with Human PCNA Protein (His Tag). Then blocked with BSA and incubated with MOB-096LC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-CD27 Recombinant Antibody (MOB-096LC) in WB
Western blot analysis of MOB-096LC was performed with Human CD27 Protein (His Tag) in reduced condition Lane 2 and Lane 3 (0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MOB-096LC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-CD27 Recombinant Antibody (MOB-096LC) in DB
Dot Blot analysis of MOB-096LC was performed by coating with Human CD27 Protein (His Tag).
MOB-096LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control, PC: Positive control
Specifications
- Immunogen
- hCD27-Fc fusion protein
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human CD27
- Species Reactivity
- Human
- Applications
- ELISA, FuncS, WB
- Related Disease
- Lymphoma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD27; CD27 molecule; T14; S152; Tp55; TNFRSF7; S152. LPFS2
- Gene ID
- 939
- UniProt ID
- P26842
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H74 | Human Anti-Human CD27 Recombinant Antibody (TAB-H74) | ELISA, IP, FC, FuncS, Neut, IF, ICC | IgG1 - kappa |
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