Human Anti-CD27 Recombinant Antibody (MOB-096LC) (CAT#: MOB-096LC)
This product is a recombinant human antibody that recognizes human CD27. The antibody was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 1 Anti-CD27 Recombinant Antibody (MOB-096LC) in ELISA
ELISA analysis of MOB-096LC was performed by coating with Human PCNA Protein (His Tag). Then blocked with BSA and incubated with MOB-096LC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-CD27 Recombinant Antibody (MOB-096LC) in WB
Western blot analysis of MOB-096LC was performed with Human CD27 Protein (His Tag) in reduced condition Lane 2 and Lane 3 (0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MOB-096LC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-CD27 Recombinant Antibody (MOB-096LC) in DB
Dot Blot analysis of MOB-096LC was performed by coating with Human CD27 Protein (His Tag).
MOB-096LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control, PC: Positive control
Specifications
- Immunogen
- hCD27-Fc fusion protein
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Human CD27
- Species Reactivity
- Human
- Applications
- ELISA, FuncS, WB
- Related Disease
- Lymphoma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD27; CD27 molecule; T14; S152; Tp55; TNFRSF7; S152. LPFS2
- Gene ID
- 939
- UniProt ID
- P26842
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2370z | Mouse Anti-CD27 Recombinant Antibody (clone 16G8) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MRO-0291-CN | Rabbit Anti-CD27 Recombinant Antibody (clone JB40-98) | WB, IF, IHC, FC | Rabbit IgG |
| FN-229CQ | Hamster Anti-CD27 Recombinant Antibody (clone LG.3A10) | FC, FuncS, IHC-Fr | Armenian hamster IgG |
| FAMAB-1626CQ | Human Anti-CD27 Recombinant Antibody (clone RM27-3E5) | IP, Neut, FC | Human IgG1 |
| VS-0824-YJ44 | Mouse Anti-CD27 Recombinant Antibody (VS-0824-YJ44) | Neut | Mouse IgG1 kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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