Human Anti-Human CD27 Recombinant Antibody (TAB-H74) (CAT#: TAB-H74)

Figure 5 Induced proliferations of T cell subsets and coexpression of costimulatory and coinhibitory receptors (immune checkpoint molecules).

Differences in short term (3 day) or long term (7 day) T cell cultures undergoing stimulations with varlilumab or control antibody (hIgG) show more CD8+ cells (a) entering the division cycle in the varlilumab-treated group. Numbers in the histograms refer to the percent of indicated cells (CD8+ or CD3+CD8-) that are dividing as reflected by CFSE staining (a bottom panel).

Ramakrishna, V., Sundarapandiyan, K., Zhao, B., Bylesjo, M., Marsh, H. C., & Keler, T. (2015). Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab. Journal for immunotherapy of cancer, 3(1), 37.

Figure 6 Induced proliferations of T cell subsets and coexpression of costimulatory and coinhibitory receptors (immune checkpoint molecules).

CD27+ proliferating CD4+ and CD8+ T cells were stained with antibodies to additional cell surface markers representing costimulatory (GITR, OX40, ICOS and 4-1BB) and coinhibitory (PD-1) molecules. Dividing CD8+ and CD4+ T cells show marked upregulation of costimulatory and coinhibitory molecules relative to non-dividing cells. Shaded histograms represent T cells stimulated with control antibody and anti-CD3 (OKT3). Numbers in the histograms refer to percent of T cells (dividing or non-dividing CD8 or CD4 cells) that are positive for the indicated costimulatory or coinhibitory marker.

Ramakrishna, V., Sundarapandiyan, K., Zhao, B., Bylesjo, M., Marsh, H. C., & Keler, T. (2015). Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab. Journal for immunotherapy of cancer, 3(1), 37.

Figure 7 Early detection of cytokines by varlilumab costimulated T cell subsets.

T cells were preactivated with OKT3 for 46 h and post activated for 4 h with OKT3+ varlilumab or OKT3+ hIgG. Intracellular staining for IFNγ response in different T cell subpopulations (a) Naïve CD4+ (CD45RO ̶) and memory (CD45RO+) and (b) naïve CD8+ (CD45RO ̶) and memory (CD45RO+) T cells. Cells were co-stained for the activation marker CD69. Numbers in individual plots refer to percent IFNγ+ cells.

Ramakrishna, V., Sundarapandiyan, K., Zhao, B., Bylesjo, M., Marsh, H. C., & Keler, T. (2015). Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab. Journal for immunotherapy of cancer, 3(1), 37.

Figure 1 Human CD27 Recombinant Antibody (TAB-H74) in SDS-PAGE

SDS-PAGE analysis of TAB-H74 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

Figure 2 Human CD27 Recombinant Antibody (TAB-H74) in HPLC

The purity of TAB-H74 was greater than 95% as determined by SEC-HPLC.