What is the significance of the non-fucosylated Fc region in this antibody?

The non-fucosylated Fc region significantly increases the antibody's affinity for Fc gamma receptor IIIa (FcγRIIIa) on immune effector cells like NK cells. This enhancement leads to a much stronger Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) response against CD30-positive cells compared to conventional fucosylated antibodies.

What type of cancer research is this anti-CD30 antibody best suited for?

This antibody targets CD30, which is a characteristic marker for Hodgkin's lymphoma and anaplastic large cell lymphoma. Therefore, it is ideally suited for research involving these malignancies, particularly studies focusing on ADCC-mediated tumor cell killing and the development of improved immunotherapies.

Anti-CD30 monoclonal antibody (MDX-1401)

CAT#: Gly-009CL

MDX-1401 is a fully human, non-fucosylated antibody that targets CD30, a marker for activated lymphocytes that is present on malignant cells of HL as well as other CD30-expressing cancers.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HDLM-2

Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg

Subcellular Location Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg

Subcellular Location Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg

Subcellular Location Figure 4 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg

Subcellular Location Figure 5 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg

Subcellular Location Figure 6 IF staining of human cell line K-562

Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg

Normal Tissue Figure 8 Appendix

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 9 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8

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Specifications

Type
Fully human Ab

Class
Antineoplastics; Monoclonal antibodies

Mechanism of action
CD30 antigen inhibitors; Immunostimulants

References

Synonyms
Lymphoma therapies; MDX-1401

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Breast cancer biomarkers at key points during disease progression

Enhanced ADCC Activity

The non-fucosylated nature of this antibody clearly boosted its ADCC activity in our assays. We compared it to a standard anti-CD30 antibody, and this product elicited a much stronger NK cell response against the lymphoma cell lines we tested.

Consistent Batch Quality

We have ordered this antibody multiple times for our long-term study on Hodgkin's lymphoma markers. The batch-to-batch consistency has been excellent, ensuring that our functional assay results remain comparable over the course of the project.

Q&As

What is the significance of the non-fucosylated Fc region in this antibody?

A: The non-fucosylated Fc region significantly increases the antibody's affinity for Fc gamma receptor IIIa (FcγRIIIa) on immune effector cells like NK cells. This enhancement leads to a much stronger Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) response against CD30-positive cells compared to conventional fucosylated antibodies.
What type of cancer research is this anti-CD30 antibody best suited for?

A: This antibody targets CD30, which is a characteristic marker for Hodgkin's lymphoma and anaplastic large cell lymphoma. Therefore, it is ideally suited for research involving these malignancies, particularly studies focusing on ADCC-mediated tumor cell killing and the development of improved immunotherapies.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - Gly-009CL.pdf

MSDS

MSDS - Gly-009CL.pdf

COA

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