Human Anti-TNFRSF8 (clone Iratumumab) scFv-Fc Chimera
CAT#: VS-0125-FY77
The CD30-specific scFv-Fc antibody is an engineered protein designed to target CD30, which is a cell surface protein expressed on certain types of malignant cells, including those involved in Hodgkin lymphoma and some non-Hodgkin lymphomas. This engineered antibody combines a single-chain variable fragment (scFv) with an Fc component, which helps to enhance its stability, serum half-life, and ability to recruit immune effector functions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HDLM-2
Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg
Subcellular Location
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg
Subcellular Location
Figure 5 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg
Subcellular Location
Figure 6 IF staining of human cell line K-562
Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg
Normal Tissue
Figure 8 Appendix
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
❮
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- Iratumumab
- Applications
- ELISA, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The function of the anti-CD30 scFv-Fc is reflected in its potential application in tumor-targeted therapy and its ability to specifically recognize CD30-positive cells.
Target
- Alternative Names
- TNF Receptor Superfamily Member 8; Lymphocyte Activation Antigen CD30; CD30L Receptor; Ki-1 Antigen; D1S166E; CD30
- Gene ID
- 943
- UniProt ID
- P28908
- Sequence Similarities
- Belongs to the TNFR8 family.
- Cellular Localization
- Cell membrane, Cytoplasm
- Post Translation Modifications
- Phosphorylated on serine and tyrosine residues.
Isoform 2 is constitutively phosphorylated.
Glycosylation at Ser206, Thr209, Asn101, and Asn276
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 9 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_011540743.1; XP_011540745.1; XP_047290749.1; XP_047290755.1; XP_054195655.1
- Function
- May play a role in the regulation of cellular growth and transformation of activated lymphoblasts.
Regulates gene expression through activation of NF-kappa-B.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-144 | Anti-Human CD30 Recombinant Antibody (Iratumumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
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| TAB-153 | Chimeric (Mouse/Human) Anti-TNFRSF8 Recombinant Antibody (TAB-153) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G062E | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G062R | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-L041E | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L041R | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-635CL | Human Anti-TNFRSF8 Recombinant Antibody (TAB-635CL) | ELISA | Human IgG |
| TAB-103LC | Anti-Human TNFRSF8 Recombinant Antibody | WB, FC, ELISA | Humanized antibody |
| TAB-103LC-S(P) | Anti-Human TNFRSF8 Recombinant Antibody scFv Fragment | WB, FC, ELISA | Humanized antibody |
| TAB-103LC-F(E) | Anti-Human TNFRSF8 Recombinant Antibody Fab Fragment | WB, FC, ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-139LC | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-139LC-1 | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0597MZ | Chicken Anti-TNFRSF8 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2165CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone mCD30.1) | FC, Stim, Costim, Block | Hamster IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2166CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone 2SH12-5F) | Neut, FC | Hamster IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3617 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3617AB) | WB, IHC-P | Rabbit IgG |
| MOR-3618 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3618AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0329-YC | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0329-YC) | ELISA, FC, Cyt, FuncS, Inhib | Mouse IgG |
| HPAB-0330-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0330-YC) | ELISA, FC, Cyt, FuncS, Inhib | Chimeric (mouse/human) IgG1, κ |
| HPAB-0331-YC | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0331-YC) | ELISA, FC, Cyt, FuncS, Inhib | Mouse IgG |
| HPAB-0332-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0332-YC) | FC, Cyt, FuncS | Human IgG1, κ |
| HPAB-0333-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0333-YC) | FC, Cyt, FuncS | Human IgG, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0445-CN-S(P) | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T105); scFv Fragment | WB, ELISA, FC | Mouse scFv |
| HPAB-0250-FY-S(P) | Recombinant Human Anti-TNFRSF8 Antibody scFv Fragment | F, IH | Human scFv |
| HPAB-1929-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 17G1); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1930-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 2H9); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1931-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 5F11); scFv Fragment | FC, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0325CQ-F(E) | Human Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0325CQ-F(E)) | ELISA, FC, FuncS, Cyt | Humanized Fab |
| HPAB-0326CQ-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody (clone Ki-4); Fab Fragment | Inhib, ELISA, FC, IF, IHC | Mouse Fab |
| HPAB-0459-WJ-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0459-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1929-FY-S(P) | Human Anti-TNFRSF8 Recombinant Antibody (clone 17G1); Fab Fragment | FC, ELISA | Human Fab |
| HPAB-1930-FY-S(P) | Human Anti-TNFRSF8 Recombinant Antibody (clone 2H9); Fab Fragment | FC, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-144 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-144) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-153 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-153) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY50 | Mouse Anti-TNFRSF8 Agonistic Antibody (VS-0724-XY50) | WB, FC, Agonistic assays, CyTOF® | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC248 | Recombinant Anti-TNFRSF8 Vesicular Antibody, EV Displayed (VS-0425-YC248) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7385 | Anti-TNFRSF8 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-YC234 | Recombinant Anti-TNFRSF8 Biparatopic Antibody, Tandem scFv | ELISA, FC, ICC, IF, IP, Neut | Tandem scFv |
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