Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA (CAT#: AGTO-G062R)
This immunotoxin was achieved by conjugating/fusing the Anti-TNFRSF8 scFv to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to TNFRSF8 overexpressed malignant lymphoma cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of malignant lymphoma.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HDLM-2
(Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg
(Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg
Figure 4 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg
Figure 5 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg
Figure 6 IF staining of human cell line K-562
(Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg
Figure 7 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg
Figure 8 Appendix
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg
Figure 9 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
Figure 9 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- Ki-3
- Molecule Class
- scFv-RTA
- Toxin Description
- Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- lymphoma
Target
- Alternative Names
- TNFRSF8; tumor necrosis factor receptor superfamily, member 8; CD30; Ki-1; D1S166E; tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; lymphocyte activation antigen CD30; Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA
- Gene ID
- 943
- UniProt ID
- A5D8T4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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