Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA

Name: Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA
SKU: AGTO-G062R
Rating: 5 (1 reviews)

CAT#: AGTO-G062R

This immunotoxin was achieved by conjugating/fusing the Anti-TNFRSF8 scFv to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to TNFRSF8 overexpressed malignant lymphoma cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of malignant lymphoma.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HDLM-2

Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg

Subcellular Location Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg

Subcellular Location Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg

Subcellular Location Figure 4 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg

Subcellular Location Figure 5 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg

Subcellular Location Figure 6 IF staining of human cell line K-562

Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg

Normal Tissue Figure 8 Appendix

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 9 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8

❮

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Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
Ki-3

Molecule Class
scFv-RTA

Toxin Description
Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
lymphoma

Target

Alternative Names
TNFRSF8; tumor necrosis factor receptor superfamily, member 8; CD30; Ki-1; D1S166E; tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; lymphocyte activation antigen CD30; Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA

Gene ID
943

UniProt ID
A5D8T4

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-G062R.pdf

MSDS

MSDS - AGTO-G062R.pdf

COA

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