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Create a titration curve to identify the best working dilution for your particular test conditions.

Can I use a secondary antibody from a different supplier?

Yes, but make sure the host species and primary antibody subclass match to assure compatibility.

What should I do if my antibody exhibits nonspecific binding?

To minimize non-specific binding, optimize the blocking environment, antibody concentration, and washing procedures.

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CD79B
Humanized Anti-CD79B Recombinant Antibody (TAB-897)

Humanized Anti-CD79B Recombinant Antibody (TAB-897) (CAT#: TAB-897)

This product is a humanized (from mouse) IgG1, κ antibody that can recognize human CD79B.

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Anti-Human CD79B Recombinant Antibody (TAB-897) in WB

Figure 1 Anti-Human CD79B Recombinant Antibody (TAB-897) in WB

Western blot analysis of TAB-897 was performed by loading human CD79B protein, His tag.

ELISA

Figure 2 Anti-Human CD79B Recombinant Antibody (TAB-897) in ELISA

Figure 2 Anti-Human CD79B Recombinant Antibody (TAB-897) in ELISA

ELISA analysis of TAB-897 was performed by coating with human CD79B protein (His tag). Then blocked with BSA and incubated with TAB-897. The HRP conjugated Anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9178/1889_C4_3_selected.jpg

Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra and weak to moderate in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9178/ihc_selected.jpg

Figure 3 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in cells of the germinal center corona.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9751/ihc_selected.jpg

Normal Tissue

Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to Nucleoplasm &
Plasma membrane & Cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9178/48_C6_1_blue_red_green.jpg

Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to Nucleoplasm &
Plasma membrane & Cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9178/47_C6_1_blue_red_green.jpg

Figure 6 Colon
(Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44107/169682_A_8_3.jpg

Figure 7 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44107/169682_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Cell line enhanced (Daudi, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000007312-CD79B

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Humanized (from mouse)

Type
Humanized (from mouse) IgG1, κ

Specificity
Human CD79B

Species Reactivity
Human

Applications
Neut, ELISA, IF, IP, FuncS, FC, ICC

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Neutralization, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation, Functional Assay, Flow Cytometry, Immunocytochemistry.

Target

Alternative Names
B29; IGB; AGM6; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; CD79b antigen (immunoglobulin-associated beta); CD79b molecule, immunoglobulin-associated beta; Ig-beta; immunoglobulin-associated B29 protein

Gene ID
974

UniProt ID
P40259

Related Resources

Biosimilar Overview
Related Signaling Pathways

Please refer to Polatuzumab Vedotin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Polatuzumab Vedotin.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD79B"

Select a product category from the dropdown menu below to view related products.

Please select product type

Rabbit Monoclonal Antibody Products MHC Tetramer Products for Cancer IgG Antibody Products ScFv Antibody Products Fab Antibody Products ADCC Enhanced Antibody Products

CAT	Product Name	Application	Type
MOR-0581	Hi-Affi™ Rabbit Anti-CD79B Recombinant Antibody (clone DS581AB)	WB, IHC, ICC, IP, FC	Rabbit IgG

CAT	Product Name	Application	Type
MHC-LC1918	PE-A*02:01/Human CD79B (LLSAEPVPA) MHC Tetramer	FCM

CAT	Product Name	Application	Type
HPAB-618-FY	Human Anti-CD79B Recombinant Antibody (HPAB-618-FY)	FC, ELISA	Human IgG
HPAB-619-FY	Human Anti-CD79B Recombinant Antibody (HPAB-619-FY)	FC, ELISA	Humanized IgG
HPAB-1491-FY	Human Anti-CD79B Recombinant Antibody (clone P2C2)	ELISA, WB	Human IgG1
HPAB-1938-FY	Mouse Anti-CD79B Recombinant Antibody (clone MA79b)	FuncS, FC	Mouse IgG1, κ
HPAB-1939-FY	Human Anti-CD79B Recombinant Antibody (clone huMA79b)	FuncS, Inhib	Humanized IgG1, κ

CAT	Product Name	Application	Type
HPAB-618-FY-S(P)	Human Anti-CD79B Recombinant Antibody; scFv Fragment (HPAB-618-FY-S(P))	FC, ELISA	Humanized scFv
HPAB-619-FY-S(P)	Human Anti-CD79B Recombinant Antibody; scFv Fragment (HPAB-619-FY-S(P))	FC, ELISA	Humanized scFv
HPAB-N0044-YC-S(P)	Human Anti-CD79b Recombinant Antibody; scFv Fragment (HPAB-N0044-YC-S(P))	FuncS	Humanized scFv
HPAB-1491-FY-F(E)	Human Anti-CD79B Recombinant Antibody (clone P2C2); scFv Fragment	ELISA, WB	Human scFv
HPAB-1939-FY-S(P)	Human Anti-CD79B Recombinant Antibody (clone huMA79b); scFv Fragment	ELISA, FC, FuncS	Humanized scFv

CAT	Product Name	Application	Type
HPAB-618-FY-F(E)	Human Anti-CD79B Recombinant Antibody; Fab Fragment (HPAB-618-FY-F(E))	FC, ELISA	Humanized Fab
HPAB-619-FY-F(E)	Human Anti-CD79B Recombinant Antibody; Fab Fragment (HPAB-619-FY-F(E))	FC, ELISA	Humanized Fab
HPAB-1059-FY-S(P)	Mouse Anti-CD79B Recombinant Antibody; scFv Fragment (HPAB-1059-FY-S(P))	FC	Mouse scFv
HPAB-1938-FY-F(E)	Mouse Anti-CD79B Recombinant Antibody (clone MA79b); Fab Fragment	ELISA, FC, FuncS	Mouse Fab, κ
HPAB-1939-FY-F(E)	Human Anti-CD79B Recombinant Antibody (clone huMA79b); Fab Fragment	ELISA, FC, FuncS	Humanized Fab, κ

CAT	Product Name	Application	Type
AFC-TAB-897	Afuco™ Anti-CD79B ADCC Recombinant Antibody (Polatuzumab), ADCC Enhanced	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody

Customer Reviews and Q&As

Customer Review Q&As

Affordably Priced for Quality
Quality meets the expectation without breaking the bank. Good value for the price.
Reproducible Across Multiple Hosts
Tested secondary antibodies from different suppliers and found it universally compatible, maintaining specificity.
Fast Delivery and Responsive Support
Shipped quickly, and customer support was immediately available to answer my storage buffer questions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-897, RRID: AB_3112017)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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