Rat Anti-CD8B Recombinant Antibody (clone 53-5.8)
CAT#: NEUT-435CQ
This antibody is a rat monoclonal antibody that binds specifically to mouse CD8β (Lyt 3.2), and it can neutralize the bioactivity of mouse CD8β (Lyt 3.2).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29164/1526_E8_1_selected.jpg
Normal Tissue
Figure 2 High expression in lymph node.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8_val_selected.jpg
Normal Tissue
Figure 3 Low expression in liver.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_4_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172116-CD8B
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Specifications
- Host Species
- Rat
- Type
- Rat IgG1
- Specificity
- Mouse CD8B
- Species Reactivity
- Mouse
- Clone
- 53-5.8
- Applications
- Depletion, Block, IF
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0012-CN-S(P) | Mouse Anti-CD8B Recombinant Antibody (clone EP42); scFv Fragment | FC | Mouse scFv |
| HPAB-S0182-YC-S(P) | Rat Anti-Cd8b1 Recombinant Antibody (clone YTS156.7.7); scFv Fragment | Neut | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0012-CN-F(E) | Mouse Anti-CD8B Recombinant Antibody (clone EP42); Fab Fragment | FC | Mouse Fab |
| HPAB-S0182-YC-F(E) | Rat Anti-Cd8b1 Recombinant Antibody (clone YTS156.7.7); Fab Fragment | Neut | Rat Fab |
| CAT | Product Name | Application | Type |
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| VS-0225-XY80 | CytoStream™ Mouse Anti-CD8B Recombinant Antibody (clone 2ST8.5H7) | FC | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY185 | Rat Anti-CD8B (clone YTS156.7.7) scFv-Fc Chimera | FC | Rat IgG2b, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1242 | Anti-CD8B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1243 | Anti-Human CD8B Immunohistochemistry Kit | IHC | |
| VS-0525-XY1244 | Anti-Rat CD8B Immunohistochemistry Kit | IHC |
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