Rat Anti-CD8B (clone YTS156.7.7) scFv-Fc Chimera (CAT#: VS-0325-FY185)
The anti-CD8B scFv-Fc antibody targets CD8β (CD8B), a co-receptor on cytotoxic T cells that enhances T cell receptor signaling. This antibody, combining scFv specificity with Fc stability, serves as a valuable tool for studying CD8B's role in T cell activation, immune response, and potential applications in immunotherapy.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29164/1526_E8_1_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/29164/1526_E8_1_selected.jpg
Figure 2 High expression in lymph node.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8_val_selected.jpg
Figure 3 Low expression in liver.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_4_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_4_val_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4353/165972_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enriched (RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172116-CD8B
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000172116-CD8B
Specifications
- Immunogen
- Mouse CD8
- Host Species
- Rat
- Type
- Rat IgG2b, scFv-Fc
- Specificity
- Mouse CD8B
- Species Reactivity
- Mouse
- Clone
- YTS156.7.7
- Applications
- Flow Cytometry
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Flow Cytometry: ≤ 0.25 µg per 10⁶ cells in 100 µl volume
Target
- Alternative Names
- CD8b Molecule; CD8 Antigen, Beta Polypeptide 1 (P37); CD8B1; T Lymphocyte Surface Glycoprotein Beta Chain; T-Cell Surface Glycoprotein CD8 Beta Chain; CD8b Antigen
- Gene ID
- 926
- UniProt ID
- P10966
- Long Name
- CD8b Molecule
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated as a consequence of T-cell activation.
Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers.
Glycosylation at Asn102
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site
- Protein Refseq
- XP_011531466.1; XP_054200532.1; NP_001171571.1; NP_004922.1; NP_742099.1
- Function
- Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses.
In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex.
The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins.
Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs).
In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.
Recommended Products
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1604MZ | Recombinant Mouse Anti-Rat CD8B Antibody (clone 452) | FC, IHC-P, IP, WB | Mouse antibody |
| FAMAB-0012-CN | Mouse Anti-CD8B Recombinant Antibody (clone EP42) | FC | Mouse IgG |
| FN-198CQ | Mouse Anti-CD8B Recombinant Antibody (clone 341) | Neut, FC, FuncS, IHC-P | Mouse IgG1, κ |
| HPAB-S0182-YC | Rat Anti-Cd8b1 Recombinant Antibody (clone YTS156.7.7) | Neut | Rat IgG |
| FAMAB-1681CQ | Mouse Anti-CD8B Recombinant Antibody (clone YTS 156.7.7) | FC, IHC | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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