Recombinant Mouse Anti-DDR1 Antibody (3E3) (CAT#: PABX-043)

Figure 1 The Anti-DDR1 mAbs Bind to the DS-Like Domain and Do Not Inhibit Ligand Binding

(A) ELISA showing binding of the indicated anti-DDR1 mAbs to recombinant DDR proteins immobilized on 96-well plates. Shown is a representative of three independent experiments, each performed in duplicate.

Carafoli, F., Mayer, M. C., Shiraishi, K., Pecheva, M. A., Chan, L. Y., Nan, R., ... & Hohenester, E. (2012). Structure of the discoidin domain receptor 1 extracellular region bound to an inhibitory Fab fragment reveals features important for signaling. Structure, 20(4), 688-697.

Figure 2 The Anti-DDR1 mAbs Block Collagen-Induced DDR1 Phosphorylation

DDR1b was transiently expressed in HEK293 cells, and the cells were stimulated with 10 μg/ml collagen I in the absence or presence of 10 μg/ml of the indicated anti-DDR1 mAbs. Aliquots of cell lysates were analyzed by SDS-PAGE and western blotting. The blots were probed with anti-phosphotyrosine (anti-PY) mAb 4G10 (upper blot) and reprobed with anti-DDR1 Ab (lower blot). Control, mouse IgG1 isotype control Ab. The experiment was performed three times with similar results.

Carafoli, F., Mayer, M. C., Shiraishi, K., Pecheva, M. A., Chan, L. Y., Nan, R., ... & Hohenester, E. (2012). Structure of the discoidin domain receptor 1 extracellular region bound to an inhibitory Fab fragment reveals features important for signaling. Structure, 20(4), 688-697.