Recombinant Mouse Anti-DDR1 Antibody (3E3) (CAT#: PABX-043)
Anti-DDR1 antibody is a Mouse antibody of IgG1 class that binds to an DDR1.
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ELISA
Figure 1 The Anti-DDR1 mAbs Bind to the DS-Like Domain and Do Not Inhibit Ligand Binding
(A) ELISA showing binding of the indicated anti-DDR1 mAbs to recombinant DDR proteins immobilized on 96-well plates. Shown is a representative of three independent experiments, each performed in duplicate.
Carafoli, F., Mayer, M. C., Shiraishi, K., Pecheva, M. A., Chan, L. Y., Nan, R., ... & Hohenester, E. (2012). Structure of the discoidin domain receptor 1 extracellular region bound to an inhibitory Fab fragment reveals features important for signaling. Structure, 20(4), 688-697.
Block
Figure 2 The Anti-DDR1 mAbs Block Collagen-Induced DDR1 Phosphorylation
DDR1b was transiently expressed in HEK293 cells, and the cells were stimulated with 10 μg/ml collagen I in the absence or presence of 10 μg/ml of the indicated anti-DDR1 mAbs. Aliquots of cell lysates were analyzed by SDS-PAGE and western blotting. The blots were probed with anti-phosphotyrosine (anti-PY) mAb 4G10 (upper blot) and reprobed with anti-DDR1 Ab (lower blot). Control, mouse IgG1 isotype control Ab. The experiment was performed three times with similar results.
Carafoli, F., Mayer, M. C., Shiraishi, K., Pecheva, M. A., Chan, L. Y., Nan, R., ... & Hohenester, E. (2012). Structure of the discoidin domain receptor 1 extracellular region bound to an inhibitory Fab fragment reveals features important for signaling. Structure, 20(4), 688-697.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Human DDR1
- Species Reactivity
- Human
- Clone
- 3E3
- Applications
- WB, Neut, FuncS, ELISA, Block
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The DDR1 antibody has been reported in applications of Western Blot, Neutralization, Functional Assay, Enzyme-linked Immunosorbent Assay, Blocking.
Target
- Alternative Names
- DDR1; discoidin domain receptor tyrosine kinase 1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; epithelial discoidin domain-containing receptor 1; tyrosine kinase DDR; cell adhesion kinase; mammary carcinoma kinase 10; tyrosine-protein kinase CAK; protein-tyrosine kinase RTK-6; neuroepithelial tyrosine kinase; PTK3A protein tyrosine kinase 3A; CD167 antigen-like family member A; neurotrophic tyrosine kinase, receptor, type 4;
- Gene ID
- 780
- UniProt ID
- Q08345
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-436 | Mouse Anti-DDR1 Recombinant Antibody (clone mAb 3E3) | ELISA, FC | Mouse IgG |
| VS3-XY571 | Mouse Anti-DDR1 Recombinant Antibody (clone 2G4E12) | ELISA, WB | Mouse IgG1 |
| VS3-WK1567 | Rabbit Anti-DDR1 Polyclonal Antibody | WB, ELISA | Rabbit IgG |
| VS-0723-WK89 | Human Anti-DDR1 Recombinant Antibody (VS-0723-WK89) | FC | Chimeric (rabbit/human) IgG1 |
| VS3-WK2188 | Rabbit Anti-DDR1 (pY513) Recombinant Antibody (VS3-WK2188) | WB, ELISA | Rabbit IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABX-043, RRID: AB_3111694)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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