Human Anti-FGFR4 Recombinant Antibody (clone XPA.48.148) (CAT#: HPAB-0058-LS)
The antibody is used to bind the FGF receptor 4 (FGFR4) and the medical use thereof, in particular for the diagnosis prevention or treatment of diseases associated with FGFR expression, overexpression or hyperactivity.
Specific Inquiry
FC
Figure 1 Three examples of anti-FGFR4 antibodies bind specifically to FGFR4.
The binding of antibodies XPA.48.056, XPA.48.117, and XPA.48.148 was evaluated by measuring binding to CHO-hFGFR4 cells (Panel A); HepG2 (Panel B); and HuH7 cells (Panel C) by FACS.
Neut
Figure 2 Three examples of anti-FGFR4 antibodies are capable of neutralizing FGF-19 induced phosphorylation of intracellular proteins.
Each of the three example antibodies were able to neutralize pERK (FIG. 4, Panel A) and pGSK-3P (FIG. 4, Panel B) in the presence of a sub-maximal concentration of rfiFGF-19 as measured using electrochemiluminescence.
ADCC
Figure 3 Three examples of anti-FGFR4 antibodies are capable of mediating antibody-dependent cellular cytotoxicity in HepG2 cells (Panel A), HuH-7 cells (Panel B) and HCT116-FGFR4 cells (Panel C).
Percent specific cytotoxicity was calculated using 1 or 10 mg/ml antibody.
FC
Figure 4 Three examples of anti-FGFR4 antibodies are capable of binding to mouse FGFR4 in addition to human FGFR4.
Panel A shows dose-dependent binding of XPA.48.056, XPA.48.117, and XPA.48.148 to endogenous AML12 mouse hepatocytes as measured by flow cytometry. Panel B shows FACS binding of 50 nM XPA.48.056, XPA.48.117, and XPA.48.148 to HEK293E cells transiently transfected with mouse FGFR4 cDNA. Data was normalized to the degree of signal measured for untransfected cells.
FuncS
Figure 5 Three examples of anti-FGFR4 antibodies are capable of inhibiting tumor growth in the HuH-7 xenograft model.
Antibodies were administered at 30 mg/kg/week for a total of 4 weeks (15 mice per group).
Specifications
- Immunogen
- FGFR4
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Human FGFR4
- Species Reactivity
- Human, Mouse
- Clone
- XPA.48.148
- Applications
- Inhib, ELISA, WB
- Related Disease
- Hepatocellular cancer, Breast cancer, Prostate cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, Neutralization, ADCC, Functional Assay.
Target
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3383z | Mouse Anti-FGFR4 Recombinant Antibody (clone 31F7) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-0739CT | Recombinant Mouse anti-Human FGFR4 Monoclonal antibody (NN0390-7I44) | WB | |
| HPAB-2922LY | Mouse Anti-FGFR4 Recombinant Antibody (HPAB-2922LY) | ELISA, FC | Mouse IgG |
| HPAB-0442-FY | Human Anti-FGFR4 Recombinant Antibody (HPAB-0442-FY) | Inhib | Human IgG |
| VS1-YJ59 | Mouse Anti-FGFR4 Recombinant Antibody (clone 19H3) | WB, IP | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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